Fussing V, Nielsen J P, Bisgaard M, Meyling A
Danish Veterinary Laboratory, Cophenhagen.
Vet Microbiol. 1999 Feb 23;65(1):61-74. doi: 10.1016/s0378-1135(98)00288-0.
The aim of the present study was to evaluate capsular-typing, plasmid-profiling, phage-typing and ribotyping for epidemiological studies of toxin-producing Pasteurella multocida ssp. multocida in Denmark. The evaluation of methods was based on 68 strains from nasal swabs and 14 strains from pneumonic lungs. Strains from lungs were all of capsular Type A, whereas strains from nasal swabs were of both capsular Types A and D. Only 9% of the strains contained plasmids, which could not be associated with antibiotic resistance. Phage-typing divided 61% of strains into 10 groups, while 39% were non-typable. CfoI ribotyping divided strains into four groups of which one type contained 94% of isolates. HindIII ribotyping divided strains into 18 types. A total of 18 strains from The Netherlands, UK and USA were subjected to HindIII ribotyping, resulting in 13 types of which six were identical to ribotypes of Danish strains. Phage-typing of isolates from an outbreak of atrophic rhinitis involving six herds in 1985 showed the existence of an epidemic strain. This type was recognised in the herd suspected of being the source of the infections and in four of the five infected herds. These findings were supported by HindIII ribotyping, as 85% of isolates from all herds were assigned to one ribotype. In conclusion, HindIII ribotyping seems to represent a useful tool for epidemiological studies of toxigenic P. multocida ssp. multocida.
本研究的目的是评估荚膜分型、质粒谱分析、噬菌体分型和核糖体分型，用于丹麦产毒素多杀性巴氏杆菌多杀亚种的流行病学研究。方法评估基于68株鼻拭子菌株和14株肺炎肺组织菌株。肺组织菌株均为荚膜A型，而鼻拭子菌株有荚膜A型和D型。仅9%的菌株含有质粒，且与抗生素耐药性无关。噬菌体分型将61%的菌株分为10组，39%不可分型。CfoI核糖体分型将菌株分为四组，其中一组包含94%的分离株。HindIII核糖体分型将菌株分为18种类型。对来自荷兰、英国和美国的18株菌株进行HindIII核糖体分型，结果有13种类型，其中6种与丹麦菌株的核糖体类型相同。对1985年涉及6个猪群的萎缩性鼻炎暴发分离株进行噬菌体分型，显示存在一种流行菌株。在疑似感染源的猪群以及5个感染猪群中的4个中识别出了这种类型。这些发现得到了HindIII核糖体分型的支持，因为所有猪群分离株的85%被归为一种核糖体类型。总之，HindIII核糖体分型似乎是产毒素多杀性巴氏杆菌多杀亚种流行病学研究的一种有用工具。
