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Changing the enantioselectivity of enzymes by directed evolution.

作者信息

Reetz Manfred T

机构信息

Max-Planck-Institut für Kohlenforschung, Mülheim An Der Ruhr, Germany.

出版信息

Methods Enzymol. 2004;388:238-56. doi: 10.1016/S0076-6879(04)88021-2.

DOI:10.1016/S0076-6879(04)88021-2

PMID:15289076

Abstract

摘要

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Changing the enantioselectivity of enzymes by directed evolution.

作者信息

Reetz Manfred T

机构信息

Max-Planck-Institut für Kohlenforschung, Mülheim An Der Ruhr, Germany.

出版信息

Methods Enzymol. 2004;388:238-56. doi: 10.1016/S0076-6879(04)88021-2.

DOI:10.1016/S0076-6879(04)88021-2

PMID:15289076

Abstract

摘要

相似文献

Changing the enantioselectivity of enzymes by directed evolution.

Methods Enzymol. 2004;388:238-56. doi: 10.1016/S0076-6879(04)88021-2.

An overview of high-throughput screening systems for enantioselective enzymatic transformations.

Methods Mol Biol. 2003;230:259-82. doi: 10.1385/1-59259-396-8:259.

Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.

J Am Chem Soc. 2010 Jul 7;132(26):9144-52. doi: 10.1021/ja1030479.

Biological chemistry: enzymes in focus.

Nature. 2005 Aug 25;436(7054):1096-7. doi: 10.1038/4361096a.

Directed evolution of lipases and esterases.

Methods Enzymol. 2004;388:199-207. doi: 10.1016/S0076-6879(04)88018-2.

Knowledge-guided laboratory evolution of protein thermolability.

Biotechnol Bioeng. 2009 Apr 15;102(6):1712-7. doi: 10.1002/bit.22202.

Directed evolution of an enantioselective lipase.

Chem Biol. 2000 Sep;7(9):709-18. doi: 10.1016/s1074-5521(00)00015-6.

Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis.

Chemphyschem. 2011 Jun 6;12(8):1550-7. doi: 10.1002/cphc.201100031. Epub 2011 Apr 5.

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.

Nat Protoc. 2007;2(4):891-903. doi: 10.1038/nprot.2007.72.

Learning from directed evolution: theoretical investigations into cooperative mutations in lipase enantioselectivity.

Chembiochem. 2004 Feb 6;5(2):214-23. doi: 10.1002/cbic.200300731.