原发性眼内淋巴瘤病例中的双侧相同单克隆性。

Bilaterally identical monoclonality in a case of primary intraocular lymphoma.

作者信息

Higashide Tomomi, Takahira Masayuki, Okumura Hirokazu, Torisaki Makoto, Sakurai Mayumi, Shirao Yutaka, Sugiyama Kazuhisa

机构信息

Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.

出版信息

Am J Ophthalmol. 2004 Aug;138(2):306-8. doi: 10.1016/j.ajo.2004.03.003.

DOI:10.1016/j.ajo.2004.03.003

PMID:15289150

Abstract

PURPOSE

To report a case of primary intraocular lymphoma possessing bilaterally identical monoclonal rearrangements of the immunoglobulin heavy chain (IgH) gene.

DESIGN

Observational case report.

METHODS

A 78-year-old woman with bilateral vitritis, subretinal infiltrates in the right eye, and no involvement of the central nervous system (CNS) was diagnosed histologically with diffuse large B-cell lymphoma by a transvitreal subretinal biopsy of the right eye. One month later, vitrectomy was performed on the left eye due to increased vitreous opacity. DNA was extracted from a vitrectomy specimen from each eye, and the third complementarity-determining region of IgH gene was analyzed by polymerase chain reaction and direct sequencing.

RESULTS

Monoclonal rearrangements of IgH gene with identical DNA sequence were detected in both eyes.

CONCLUSIONS

Bilaterally identical monoclonality was detected in a case of primary intraocular lymphoma with no CNS involvement.

摘要

目的

报告一例原发性眼内淋巴瘤病例，其免疫球蛋白重链（IgH）基因存在双侧相同的单克隆重排。

设计

观察性病例报告。

方法

一名78岁女性，患有双侧葡萄膜炎，右眼有视网膜下浸润，且中枢神经系统（CNS）未受累，通过右眼经玻璃体视网膜下活检组织学诊断为弥漫性大B细胞淋巴瘤。1个月后，由于左眼玻璃体混浊加重，对其进行了玻璃体切除术。从每只眼的玻璃体切除标本中提取DNA，并通过聚合酶链反应和直接测序分析IgH基因的第三个互补决定区。