通过染色体显微切割和荧光原位杂交技术对韩国田鼠（姬鼠属，鼠亚科）的微观和宏观B染色体进行比较分析。

Comparative analysis of micro and macro B chromosomes in the Korean field mouse Apodemus peninsulae (Rodentia, Murinae) performed by chromosome microdissection and FISH.

作者信息

Rubtsov N B, Karamysheva T V, Andreenkova O V, Bochkaerev M N, Kartavtseva I V, Roslik G V, Borissov Y M

机构信息

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

出版信息

Cytogenet Genome Res. 2004;106(2-4):289-94. doi: 10.1159/000079301.

DOI:10.1159/000079301

PMID:15292605

Abstract

Comparative analysis of micro B and macro B chromosomes of the Korean field mouse Apodemus peninsulae, collected in populations from Siberia and the Russian Far East, was performed with Giemsa, DAPI, Ag-NOR staining and chromosome painting with whole and partial chromosome probes generated by microdissection and DOP-PCR. DNA composition of micro B chromosomes was different from that of macro B chromosomes. All analyzed micro B chromosomes contained clusters of DNA repeats associated with regions characterized by an uncondensed state in mitosis. Giemsa and DAPI staining did not reveal these regions. Their presence in micro B chromosomes led to their special morphology and underestimation in size. DNA repeat clusters homologous to DNA of micro B chromosome arms were also revealed in telomeric regions of some macro B chromosomes of specimens captured in Siberian regions. Neither active NORs nor clusters of ribosomal DNA were found in the uncondensed regions of micro B chromosomes. Possible evolutionary pathways for the origin of macro and micro B chromosomes are discussed.

摘要

对采自西伯利亚和俄罗斯远东地区种群的半岛田鼠的微小B染色体和巨大B染色体进行了比较分析，采用吉姆萨染色、DAPI染色、银染核仁组织区（Ag-NOR）染色以及使用通过显微切割和DOP-PCR产生的全染色体和部分染色体探针进行染色体描绘。微小B染色体的DNA组成与巨大B染色体不同。所有分析的微小B染色体都含有与有丝分裂中以解聚状态为特征的区域相关的DNA重复序列簇。吉姆萨染色和DAPI染色未显示这些区域。它们在微小B染色体中的存在导致了其特殊的形态和大小被低估。在西伯利亚地区捕获的一些标本的巨大B染色体的端粒区域也发现了与微小B染色体臂的DNA同源的DNA重复序列簇。在微小B染色体的解聚区域未发现活跃的核仁组织区（NOR）或核糖体DNA簇。讨论了巨大B染色体和微小B染色体起源的可能进化途径。