Rubtsov N B, Karamysheva T V, Bogdanov A S, Likhoshvaĭ T V, Kartavtseva I V
Genetika. 2011 Sep;47(9):1236-46.
The homology of DNA of C-positive centromeric regions of chromosomes in wood mice of the genus Sylvaemus (S. uralensis, S. fulvipectus, S. sylvaticus, S. flavicollis, and S. ponticus) was estimated for the first time. DNA probes were generated by microdissection from the centromeric regions of individual autosomes of each species, and their fluorescence in situ hybridization (FISH) with metaphase chromosomes of representatives of all studied wood mouse species was carried out. Unlike in the chromosomal forms and races of S. uralensis, changes in the DNA composition of the chromosomal centromeric regions in the wood mouse species of the genus Sylvaemus (including closely related S. flavicollis and S. ponticus) are both quantitative and qualitative. The patterns of FISH signals after in situ hybridization of the microdissection DNA probes with chromosomes of the species involved in the study demonstrate significant differences between C-positive regions of wood mouse chromosomes in the copy number and the level of homology of repetitive sequences as well as in the localization of homologous repetitive sequences. It was shown that C-positive regions of wood mouse chromosomes can contain both homologous and distinct sets of repetitive sequences. Regions enriched with homologous repeats were detected either directly in C-positive regions of individual chromosomes or only on the short arms of acrocentrics, or at the boundary of C-positive and C-negative regions.
首次对林姬鼠属（乌拉尔林姬鼠、黄胸林姬鼠、森林林姬鼠、黄颈林姬鼠和 Ponticus 林姬鼠）的木鼠染色体 C 阳性着丝粒区域的 DNA 同源性进行了评估。通过显微切割从每个物种的单个常染色体的着丝粒区域生成 DNA 探针，并将其与所有研究的木鼠物种代表的中期染色体进行荧光原位杂交（FISH）。与乌拉尔林姬鼠的染色体形式和种族不同，林姬鼠属的木鼠物种（包括亲缘关系较近的黄颈林姬鼠和 Ponticus 林姬鼠）染色体着丝粒区域的 DNA 组成变化既有数量上的，也有质量上的。显微切割 DNA 探针与参与研究的物种染色体进行原位杂交后的 FISH 信号模式表明，木鼠染色体的 C 阳性区域在重复序列的拷贝数、同源性水平以及同源重复序列的定位方面存在显著差异。结果表明，木鼠染色体的 C 阳性区域可能同时包含同源和不同的重复序列集。富含同源重复序列的区域要么直接在单个染色体的 C 阳性区域中检测到，要么仅在近端着丝粒染色体的短臂上检测到，要么在 C 阳性和 C 阴性区域的边界处检测到。
