Pearson M, Bjornson R, Pearson G, Rohrmann G
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Science. 1992 Sep 4;257(5075):1382-4. doi: 10.1126/science.1529337.
The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV), which is used for the overexpression of eukaryotic genes and is being engineered for possible use as a viral insecticide, has a circular, supercoiled genome of approximately 128 kilobases. Despite its widespread use, little is known about the mechanism by which AcMNPV replicates. Evidence is presented in this report that AcMNPV origins of DNA replication are repeated sequences each containing several closely related imperfect palindromes that are present in six regions distributed around the genome. Although AcMNPV infection-dependent plasmid replication was initiated by a single complete palindrome, the amount of replication was substantially increased in plasmids containing six or eight palindromes.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）用于真核基因的过表达，并且正在被改造以可能用作病毒杀虫剂，它具有一个约128千碱基的环状、超螺旋基因组。尽管其被广泛使用，但关于AcMNPV复制的机制却知之甚少。本报告提供的证据表明，AcMNPV的DNA复制起始位点是重复序列，每个重复序列都包含几个紧密相关的不完全回文序列，这些序列存在于基因组周围分布的六个区域中。虽然依赖AcMNPV感染的质粒复制是由单个完整回文序列启动的，但在含有六个或八个回文序列的质粒中，复制量显著增加。
