Hungnes O, Tjøtta E, Grinde B
Department of Virology, National Institute of Public Health, Oslo, Norway.
Virology. 1992 Sep;190(1):440-2. doi: 10.1016/0042-6822(92)91230-r.
The reverse transcription of HIV-1 generates a linear genomic DNA with a single-stranded gap. The gap is believed to be the result of plus-strand priming from a second, centrally located, polypurine tract (PPT). A mutant containing four amino-acid-neutral purine-to-pyrimidine changes within the central PPT did not replicate as fast as wild-type virus. Another mutant with the entire 15-bp PPT deleted was replication-deficient. The results indicate that plus-strand priming at the central PPT is important for viral replication, possibly by ensuring efficient DNA synthesis.
HIV-1的逆转录产生具有单链缺口的线性基因组DNA。该缺口被认为是来自第二个位于中心位置的多聚嘌呤序列（PPT）的正链引发的结果。在中心PPT内含有四个氨基酸中性嘌呤到嘧啶变化的突变体复制速度不如野生型病毒快。另一个缺失整个15bp PPT的突变体缺乏复制能力。结果表明，中心PPT处的正链引发对病毒复制很重要，可能是通过确保有效的DNA合成来实现的。
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