Johnson James R, Owens Krista, Sabate Montse, Prats Guillem
Mucosal and Vaccine Research Center, Minneapolis VA Medical Center, 1 Veterans Dr., Minneapolis, MN 55417, USA.
J Clin Microbiol. 2004 Aug;42(8):3841-3. doi: 10.1128/JCM.42.8.3841-3843.2004.
Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.
基于在管家基因fumC中鉴定出的一种新型单核苷酸多态性(即G594A),设计了针对大肠杆菌O15:K52:H1的特异性引物。在比较各种参考分型方法的实验中,新引物对O15:K52:H1克隆群的敏感性和特异性均为100%,其中包括162株不同的临床和参考大肠杆菌分离株。