School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia.
Comp Immunol Microbiol Infect Dis. 2012 Jul;35(4):319-24. doi: 10.1016/j.cimid.2012.02.002. Epub 2012 Mar 3.
Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQ(r)) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQ(r) ExPEC population in Australia and may potentially be transferred between humans and dogs.
抗微生物药物耐药性的肠外致病性大肠杆菌（ExPEC）对人类和兽医医学都有影响。一种越来越多的多药耐药的 ExPEC 克隆群是血清型 O15:K52:H1。因此，我们在来自澳大利亚的人类（n=582）和狗（n=120）的氟喹诺酮耐药（FQ(r)）大肠杆菌临床分离株中寻找 O15:K52:H1 菌株。在 D 群的分离株（267/702；38%）中筛选 fumC 和 O15 rfb 变体的 O15:K52:H1 特异性单核苷酸多态性（SNP）。确定的 34 个 O15:K52:H1 分离株（33 个来自人类，1 个来自犬）进行了抗菌药物敏感性分析、毒力基因分型和宏限制谱分析。尽管敏感性谱有所不同，但 34 个分离株通过脉冲场凝胶电泳密切相关，并表现出典型的 O15:K52:H1 相关毒力谱（完整的 pap 操纵子、F16 papA 等位基因、papG 等位基因 II、iha、fimH、sat、fyuA、iutA、kpsMII、ompT）。犬分离株与人类分离株非常相似。因此，O15:K52:H1 菌株在澳大利亚的 FQ(r) ExPEC 人群中起作用，并且可能在人类和狗之间转移。
