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红酵母嗜鲑色掷孢酵母醛还原酶的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction study of aldehyde reductase from a red yeast, Sporobolomyces salmonicolor.

作者信息

Kataoka M, Yamamoto K, Shimizu S, Kita K, Hashimoto T, Yanase H, Kita A, Miki K

机构信息

Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.

出版信息

Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):405-6. doi: 10.1107/S0907444995011012.

Abstract

Crystals of aldehyde reductase from a red yeast, Sporobolomyces salmonicolor, have been grown from an ammonium sulfate solution, pH 7.0, by means of the vapor-diffusion procedure. The crystals belong to the hexagonal system, space group P6(1)22 or its enantiomorph, P6(5)22, with unit-cell dimensions of a = 72.2 and c = 320 A. The X-ray diffraction patterns extend to at least 2 A resolution with the use of synchrotron radiation. The crystals are stable on exposure to X-rays and suitable for high-resolution X-ray structure determination.

摘要

已通过气相扩散法,从pH 7.0的硫酸铵溶液中培养出了红色酵母——深红酵母醛还原酶的晶体。这些晶体属于六方晶系,空间群为P6(1)22或其对映体P6(5)22,晶胞参数为a = 72.2 Å,c = 320 Å。利用同步辐射,X射线衍射图谱的分辨率至少达到2 Å。这些晶体在X射线下稳定,适合用于高分辨率X射线结构测定。

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