Sehnke P C, Johnson J E
Department of Horticultural Sciences, University of Florida, Gainesville 32611.
Virology. 1993 Sep;196(1):328-31. doi: 10.1006/viro.1993.1483.
Isolated tobacco streak virus strain mild (TSV-M) top nucleoprotein component (TV) was crystallized by vapor diffusion with polyethylene glycol (PEG) and methyl pentanediol. The morphology of the crystal suggested a hexagonal space group, however, the crystals were disordered as analyzed by X-ray diffraction. Capsid protein from TSV (strains M and white clover) was treated with trypsin to remove 87 amino terminal residues. The modified capsid protein was purified by ion exchange chromatography and crystallized in both hexagonal and triclinic forms. Hexagonal crystals grown by vapor diffusion in PEG diffract X rays to 4.5 A. The crystals, in the space group P6(2) or its enantiomorph, possess unit cell dimensions of a = 98.3 A, c = 108.7 A and probably contain 12 dimers/unit cell. Triclinic crystals grown by vapor diffusion in ammonium sulfate diffracted X rays to 2.4 A resolution when exposed to X-ray synchrotron radiation. The crystals have unit cell dimensions of a = 60.1 A, b = 77.5 A, c = 73.9 A with alpha = 67.8 degrees, beta = 130 degrees, and gamma = 106 degrees and probably contain 4 dimers/unit cell.
分离出的烟草线条病毒温和株（TSV-M）的顶部核蛋白组分（TV）通过与聚乙二醇（PEG）和甲基戊二醇的气相扩散法结晶。晶体形态表明为六方空间群，然而，经X射线衍射分析，晶体是无序的。用胰蛋白酶处理来自TSV（M株和白三叶草株）的衣壳蛋白以去除87个氨基末端残基。经修饰的衣壳蛋白通过离子交换色谱法纯化，并以六方和三斜两种形式结晶。通过在PEG中气相扩散生长的六方晶体对X射线的衍射分辨率为4.5埃。这些晶体属于空间群P6(2)或其对映体，晶胞参数为a = 98.3埃，c = 108.7埃，每个晶胞可能包含12个二聚体。当暴露于X射线同步辐射时，通过在硫酸铵中气相扩散生长的三斜晶体对X射线的衍射分辨率为2.4埃。这些晶体的晶胞参数为a = 60.1埃，b = 77.5埃，c = 73.9埃，α = 67.8°，β = 130°，γ = 106°，每个晶胞可能包含4个二聚体。
