在一名复发性滤泡性淋巴瘤患者的直肠小黏膜下肿瘤石蜡包埋组织切片上检测BCL2-IGH重排。
Detection of BCL2-IGH rearrangement on paraffin-embedded tissue sections obtained from a small submucosal tumor of the rectum in a patient with recurrent follicular lymphoma.
作者信息
Yoshida Naohisa, Nomura Kenichi, Matsumoto Yosuke, Nishida Kazuhiro, Wakabayashi Naoki, Konishi Hideyuki, Mitsufuji Shoji, Kataoka Keisho, Okanoue Takeshi, Taniwaki Masafumi
机构信息
Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto 602-8566, Japan.
出版信息
World J Gastroenterol. 2004 Sep 1;10(17):2602-4. doi: 10.3748/wjg.v10.i17.2602.
A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma (FL). Colonoscopic examination revealed a rectal submucosal tumor (SMT) without any erosions and ulcers. In this patient, it was difficult to distinguish non-Hodgkin's lymphoma (NHL) invasion from other disorders of the colon including carcinoid tumor merely based on endoscopic findings. Histopathologic and immunohistochemical studies on biopsy specimens showed an infiltration of atypical lymphocytes that were positive for CD20 and BCL2 but negative for UCHL-1. Fluorescence in situ hybridization on paraffin-embedded tissue sections (T-FISH) identified a translocation of BCL2 with IGH gene. Based on these findings, the tumor was defined as an invasion of FL. T-FISH method is useful for the detection of a monoclonality of atypical lymphocytes in an SMT of the gastrointestinal tract, and particularly for the detection of chromosomal translocations specific to lymphoma subtypes.
一名59岁女性因复发性滤泡性淋巴瘤（FL）入住我院。结肠镜检查发现直肠黏膜下肿瘤（SMT），无任何糜烂和溃疡。在该患者中，仅根据内镜检查结果很难将非霍奇金淋巴瘤（NHL）浸润与包括类癌肿瘤在内的结肠其他疾病区分开来。活检标本的组织病理学和免疫组织化学研究显示，非典型淋巴细胞浸润，CD20和BCL2呈阳性，但UCHL-1呈阴性。石蜡包埋组织切片的荧光原位杂交（T-FISH）检测到BCL2与IGH基因的易位。基于这些发现，该肿瘤被定义为FL浸润。T-FISH方法有助于检测胃肠道SMT中非典型淋巴细胞的单克隆性，特别是检测淋巴瘤亚型特有的染色体易位。
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