Luo Huan-liang, Guo Yong, Cui Tang-bing, Dai Jian-guo, Zhang Jun-song, Xu Bai-qiu
Food and Bioengineer College, South China University of Technology, Guangzhou 510641, China.
Yao Xue Xue Bao. 2004 Apr;39(4):305-8.
To investigate the effects of fungal elicitors on inophyllums production in suspension cultured cell of Calophyllum inophyllum Linn.
The pathogen of leaf spot disease of C. inophyllum L. was isolated and prepared as fungal elicitor. The fungal elicitor was added to the medium with different concentrations and culture period. Their effects on biomass and inophyllums content of the suspension of cultured cells were studied.
The optimum effects of S-I fungal elicitor concentrations on inophyllums content was 60 mg GE x L(-1). Adding the fungi elicitor into the cell suspension culture system at stationary phase (being cultured for 18 days) resulted in a highest inophyllum content of 59.174 mg x L(-1) at the 3rd day with 27% higher than control. Fungal elicitor treatment promoted the inophyllums accumulation in medium.
Adding the Stagonospora curtisii (Berk.) Sacc. to the medium was effective approaches to enhance inophyllums yield in the suspension of C. inophyllum L culture cell.
研究真菌激发子对红厚壳悬浮培养细胞中红厚壳素产生的影响。
分离红厚壳叶斑病病原菌并制备成真菌激发子。将真菌激发子添加到不同浓度和培养时期的培养基中。研究其对培养细胞悬浮液生物量和红厚壳素含量的影响。
S-I真菌激发子浓度对红厚壳素含量的最佳影响为60mg GE×L(-1)。在稳定期（培养18天）将真菌激发子添加到细胞悬浮培养体系中，第3天红厚壳素含量最高，为59.174mg×L(-1)，比对照高27%。真菌激发子处理促进了培养基中红厚壳素的积累。
向培养基中添加柯氏叶点霉是提高红厚壳悬浮培养细胞中红厚壳素产量的有效途径。
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