人类白细胞抗原-G抗原与分娩：孕期母体血清、胎儿血清及羊水水平

HLA-G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy.

作者信息

Hackmon Rinat, Hallak Mordechai, Krup Margalit, Weitzman Dahlia, Sheiner Eyal, Kaplan Boris, Weinstein Yacob

机构信息

Department of Obstetrics and Gynecology, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.

出版信息

Fetal Diagn Ther. 2004 Sep-Oct;19(5):404-9. doi: 10.1159/000078992.

DOI:10.1159/000078992

PMID:15305096

Abstract

OBJECTIVE

To determine whether soluble HLA-G1 (sHLA-G1) concentrations in maternal serum and in amniotic fluid are lower at term than in the second trimester.

METHODS

In this prospective study amniotic fluid and maternal serum samples were aspirated from 21 pregnant women during genetic amniocentesis at 16-20 weeks' gestation, and from 19 women undergoing a cesarean section at term. In the latter group arterial umbilical cord blood was aspirated as well. sHLA-G1 levels were determined using ELISA assay. This assay included the anti-HLA-G monoclonal antibodies 87G and 16G1, both as capture antibodies and horseradish-peroxidase-labeled rabbit anti-human beta(2)-microglobulin antibodies, as the detection antibody. The relative concentrations of sHLA-G1 were measured from the absorbancy of the blue product at 650 nm. Student's t test was used for statistical analysis.

RESULTS

sHLA-G1 levels in amniotic fluid were significantly lower at term than in the second trimester (0.160 +/- 0.05 vs. 0.272 +/- 0.150 OD units; p < 0.05). Levels of sHLA-G1 in maternal serum declined toward term, but the difference from the second trimester was not statistically significant (0.266 +/- 0.157 vs. 0.205 +/- 0.120 OD units; p = 0.193). There was a strong correlation of sHLA-G1 concentrations between cord serum and maternal serum (R(2) = 0.79; p < 0.001), but not between cord serum and amniotic fluid (R(2) = 0.00004) or amniotic fluid and maternal serum (R(2) = 0.02).

CONCLUSIONS

sHLA-G1 antigen expression is higher in amniotic fluid than in maternal-fetal compartments and significantly decreases toward term. We speculate that the declining amniotic fluid sHLA-G1 levels may stimulate a maternal immunological response against the fetus and contribute to the initiation of parturition.

摘要

目的

确定足月时母血清和羊水中可溶性 HLA - G1（sHLA - G1）浓度是否低于孕中期。

方法

在这项前瞻性研究中，从 21 名妊娠 16 - 20 周行遗传羊膜腔穿刺术的孕妇以及 19 名足月行剖宫产的妇女中采集羊水和母血清样本。在后一组中还采集了脐动脉血。使用 ELISA 测定法测定 sHLA - G1 水平。该测定法包括抗 HLA - G 单克隆抗体 87G 和 16G1，二者均作为捕获抗体，以及辣根过氧化物酶标记的兔抗人β2 - 微球蛋白抗体作为检测抗体。从 650nm 处蓝色产物的吸光度测量 sHLA - G1 的相对浓度。采用 Student's t 检验进行统计分析。

结果

足月时羊水中的 sHLA - G1 水平显著低于孕中期（0.160±0.05 对比 0.272±0.150 OD 单位；p < 0.05）。母血清中 sHLA - G1 水平足月时下降，但与孕中期的差异无统计学意义（0.266±0.157 对比 0.205±0.120 OD 单位；p = 0.193）。脐血清与母血清之间的 sHLA - G1 浓度存在强相关性（R² = 0.79；p < 0.001），但脐血清与羊水之间（R² = 0.00004）或羊水与母血清之间（R² = 0.02）无相关性。