Hackmon Rinat, Koifman Arie, Hyodo Hironobu, Glickman Hagit, Sheiner Eyal, Geraghty Daniel E
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Am J Obstet Gynecol. 2007 Sep;197(3):255.e1-5. doi: 10.1016/j.ajog.2007.06.033.
Recently, lower maternal plasma human leukocyte antigen (HLA)-G protein levels in preeclampsia (PE) in the first and second trimester was reported. Thus, we sought to evaluate the levels of HLA-G protein in patients with severe PE during the third trimester.
In this prospective case control study, amniotic fluid and maternal and cord blood samples were aspirated from 50 pregnant women during the third trimester. The study group included 26 pregnant women diagnosed with severe PE and 24 women without PE serving as controls. A soluble HLA-G-specific enzyme-linked immunosorbent assay was used to measure protein levels. Statistical analysis included the Student t test and simple regression analysis.
Maternal serum HLA-G levels in PE pregnancies were found to be significantly lower as compared with normal pregnancies (10.97 +/- 6.55 vs 36.05 +/- 34.53 microg/mL; P = .003).
A reduced level of maternal HLA-G protein was associated with severe PE during the third trimester. This finding may suggest an essential role for HLA-G in normal and preeclamptic pregnancies.
最近有报道称，妊娠早期和中期子痫前期（PE）患者母体血浆中人类白细胞抗原（HLA）-G蛋白水平较低。因此，我们试图评估妊娠晚期重度PE患者的HLA-G蛋白水平。
在这项前瞻性病例对照研究中，从50名妊娠晚期孕妇中采集羊水、母体血和脐带血样本。研究组包括26名被诊断为重度PE的孕妇，24名无PE的孕妇作为对照组。采用可溶性HLA-G特异性酶联免疫吸附试验测量蛋白水平。统计分析包括学生t检验和简单回归分析。
发现PE妊娠孕妇的母体血清HLA-G水平显著低于正常妊娠孕妇（10.97±6.55对36.05±34.53μg/mL；P = 0.003）。
母体HLA-G蛋白水平降低与妊娠晚期重度PE有关。这一发现可能提示HLA-G在正常妊娠和子痫前期妊娠中起重要作用。
