Waley S G
Laboratory of Molecular Biophysics, University of Oxford, U.K.
Biochem J. 1992 Sep 1;286 ( Pt 2)(Pt 2):357-9. doi: 10.1042/bj2860357.
The scope and limitations of a simple and satisfactory method of deducing steady-state rate equations is described. This method (called the Flux Method) consists in writing down the flux in successive steps of the reaction, and calculating the relative concentration of enzyme forms and thence the turnover time. Kinetic mechanisms for linear and branched pathways are used as examples of this method.
本文描述了一种推导稳态速率方程的简单且令人满意的方法的范围和局限性。这种方法(称为通量法)包括写出反应连续步骤中的通量,计算酶形式的相对浓度,进而计算周转时间。以线性和分支途径的动力学机制作为该方法的示例。