Stephens J W, Unruh J A, Dikeman M E, Hunt M C, Lawrence T E, Loughin T M
Departments of Animal Sciences and Industry, Kansas State University, Manhattan, KS 66506, USA.
J Anim Sci. 2004 Jul;82(7):2077-86. doi: 10.2527/2004.8272077x.
Two experiments were conducted to determine the effectiveness of using mechanical probes and objective color measurement on beef LM to predict cooked tenderness. In Exp. 1, sharp needle (SN), sharp blade (SB), blunt needle (BN), blunt blade (BB), and plumb bob (PB) probes were used to measure uncooked LM (n = 29) at 2 d postmortem in both a perpendicular and parallel orientation to the long axis of the strip loin. Additionally, instrumental color measurements were measured on uncooked muscle at 2 d postmortem. Steaks for trained sensory panel (TSP) and Warner-Bratzler shear force (WBSF) measurements were aged 14 d postmortem before cooking. Probe measurements taken perpendicular to the long axis of the LM were not correlated (P = 0.22 to 0.82) to TSP tenderness. Probe measurements (BB, BN, SN, SB, and PB) taken parallel to the long axis were correlated to TSP tenderness (r = -0.57, -0.40, -0.77, -0.52, and -0.53, respectively). A regression equation using the SN probe to predict TSP tenderness had a R2 value of 0.74. The SB probe combined with L* accounted for 45% of the variation in TSP tenderness, whereas the PB probe combined with L* accounted for 56% of the variation in TSP tenderness. A second experiment (n = 24) was conducted using the SN, SB, and PB probes on uncooked sections at 2 d and on cooked steaks at 14 d postmortem. Probe measurements on cooked steaks were not correlated to TSP tenderness. New regression equations were calculated using the probe measurements on uncooked steaks from both experiments. Prediction equations formulated with L* values and either SN, SB, or PB probes accounted for 49, 50, and 47% of the variability in TSP tenderness scores, respectively. An equation using WBSF of cooked steaks to predict TSP tenderness had an R2 of 0.58. Of the steaks predicted to be tender (predicted tenderness > 5.0) by the equations using the SN, SB, and PB probes on uncooked steaks and WBSF on cooked steaks, 85, 88, 80, and 84%, respectively, were actually tender (TSP tenderness > 5.0). Mechanical probe measurements of uncooked steaks at 2 d postmortem can potentially classify strip loins into groups based on tenderness, as well as WBSF measurements, which are more costly and time consuming.
进行了两项试验,以确定使用机械探针和客观颜色测量来预测牛肉腰大肌(LM)熟肉嫩度的有效性。在试验1中,使用尖针(SN)、尖刀片(SB)、钝针(BN)、钝刀片(BB)和铅锤(PB)探针,在宰后2天以垂直和平行于里脊长条纵轴的方向测量未烹饪的LM(n = 29)。此外,在宰后2天对未烹饪的肌肉进行仪器颜色测量。用于训练有素的感官评定小组(TSP)和沃纳-布拉茨勒剪切力(WBSF)测量的牛排,在宰后熟化14天再进行烹饪。垂直于LM纵轴进行的探针测量与TSP嫩度不相关(P = 0.22至0.82)。平行于纵轴进行的探针测量(BB、BN、SN、SB和PB)与TSP嫩度相关(r分别为-0.57、-0.40、-0.77、-0.52和-0.53)。使用SN探针预测TSP嫩度的回归方程的R2值为0.74。SB探针与L结合可解释TSP嫩度变化的45%,而PB探针与L结合可解释TSP嫩度变化的56%。进行了第二项试验(n = 24),在宰后2天对未烹饪的切片以及宰后14天对烹饪好的牛排使用SN、SB和PB探针进行测量。对烹饪好的牛排进行的探针测量与TSP嫩度不相关。使用两项试验中未烹饪牛排的探针测量值计算了新的回归方程。用L*值和SN、SB或PB探针建立的预测方程分别解释了TSP嫩度评分变异的49%、50%和47%。用烹饪好的牛排的WBSF预测TSP嫩度的方程的R2为0.58。在使用未烹饪牛排的SN、SB和PB探针以及烹饪好的牛排的WBSF的方程预测为嫩(预测嫩度>5.0)的牛排中,实际上分别有85%、88%、80%和84%是嫩的(TSP嫩度>5.0)。宰后2天对未烹饪牛排进行的机械探针测量有可能根据嫩度对里脊长条进行分组,WBSF测量也是如此,但WBSF测量成本更高且耗时更长。