大肠杆菌nusG功能对λ N介导的转录抗终止的影响。
Effect of Escherichia coli nusG function on lambda N-mediated transcription antitermination.
作者信息
Sullivan S L, Ward D F, Gottesman M E
机构信息
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032.
出版信息
J Bacteriol. 1992 Feb;174(4):1339-44. doi: 10.1128/jb.174.4.1339-1344.1992.
Abstract
The Escherichia coli Nus factors act in conjunction with the bacteriophage lambda N protein to suppress transcription termination on the lambda chromosome. NusA binds both N and RNA polymerase and may also interact with other Nus factors. To search for additional components of the N antitermination system, we isolated host revertants that restored N activity in nusA1 mutants. One revertant, nusG4, was mapped to the rif region of the E. coli chromosome and shown to represent a point mutation near the 3' end of the nusG gene. The nusG4 mutation also suppressed nusE71 but not nusASal, nusB5, nusC60 (rpoB60), or nusD026 (rho026). However, nusG+ expressed from a multicopy plasmid suppressed nusD026 and related rho mutants for both lambda and phage T4 growth. These results suggest that NusG may act as a component of the N antitermination complex. In addition, the data imply a role for NusG in Rho-dependent termination.
摘要
大肠杆菌的Nus因子与噬菌体λ N蛋白协同作用，抑制λ染色体上的转录终止。NusA既能结合N又能结合RNA聚合酶，还可能与其他Nus因子相互作用。为了寻找N抗终止系统的其他组分，我们分离了能在nusA1突变体中恢复N活性的宿主回复突变体。其中一个回复突变体nusG4被定位到大肠杆菌染色体的rif区域，并且显示它代表nusG基因3'端附近的一个点突变。nusG4突变也能抑制nusE71，但不能抑制nusASal、nusB5、nusC60（rpoB60）或nusD026（rho026）。然而，从多拷贝质粒表达的nusG+能抑制nusD026以及与λ和噬菌体T4生长相关的rho突变体。这些结果表明NusG可能作为N抗终止复合物的一个组分发挥作用。此外，这些数据暗示了NusG在依赖Rho的终止过程中的作用。
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本文引用的文献
1
Termination of transcription and its regulation in the tryptophan operon of E. coli.大肠杆菌色氨酸操纵子中的转录终止及其调控。
Cell. 1981 Apr;24(1):10-23. doi: 10.1016/0092-8674(81)90496-7.
2
Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.启动子克隆质粒的特性分析：大肠杆菌rif区域操纵子结构的分析及增强型内部启动子突变体的分离
J Bacteriol. 1980 Dec;144(3):904-16. doi: 10.1128/jb.144.3.904-916.1980.
3
Role of the host cell in bacteriophage T4 development. II. Characterization of host mutants that have pleiotropic effects on T4 growth.宿主细胞在噬菌体T4发育中的作用。II. 对T4生长具有多效性影响的宿主突变体的特征描述。
J Virol. 1980 Sep;35(3):775-89. doi: 10.1128/JVI.35.3.775-789.1980.
4
Role of the host cell in bacteriophage T4 development. I. Characterization of host mutants that block T4 head assembly.宿主细胞在噬菌体T4发育中的作用。I. 阻断T4头部组装的宿主突变体的特征分析。
J Virol. 1980 Jan;33(1):366-76. doi: 10.1128/JVI.33.1.366-376.1980.
5
The nusA gene protein of Escherichia coli. Its identification and a demonstration that it interacts with the gene N transcription anti-termination protein of bacteriophage lambda.大肠杆菌的nusA基因蛋白。其鉴定以及与噬菌体λ的基因N转录抗终止蛋白相互作用的证明。
J Mol Biol. 1981 Mar 25;147(1):11-23. doi: 10.1016/0022-2836(81)90076-0.
6
RNA polymerase pausing and transcript release at the lambda tR1 terminator in vitro.体外λ噬菌体tR1终止子处的RNA聚合酶暂停与转录物释放
J Biol Chem. 1983 Aug 10;258(15):9391-7.
7
Termination of transcription by nusA gene protein of Escherichia coli.大肠杆菌nusA基因蛋白介导的转录终止
Nature. 1981 Jul 16;292(5820):215-20. doi: 10.1038/292215a0.
8
The nus mutations affect transcription termination in Escherichia coli.nus突变影响大肠杆菌中的转录终止。
Nature. 1981 Jul 16;292(5820):212-5. doi: 10.1038/292212a0.
9
Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcription.大肠杆菌的σ因子和nusA基因蛋白在转录起始-终止循环中与RNA聚合酶的相互作用。
Cell. 1981 May;24(2):421-8. doi: 10.1016/0092-8674(81)90332-9.
10
lambda mutation in the Escherichia coli rho gene that inhibits the N protein activity of phage lambda.大肠杆菌rho基因中的λ突变抑制噬菌体λ的N蛋白活性。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5530-4. doi: 10.1073/pnas.80.18.5530.
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