Deng Zhong-Bin, Zhu Wei, Lu Chang-Ming, Shi Qin, Ju Song-Gunag, Ma Hong-Bing, Xu Yin, Zhang Xue-Guang
Institute of Biotechnology, Soochow University, Suzhou, China.
Hybrid Hybridomics. 2004 Jun;23(3):176-82. doi: 10.1089/1536859041224299.
The inducible costimulator (ICOS), the third member of the CD28/CD152 receptor family, is an important costimulatory molecule during the immune response. In this study, a functional anti-human ICOS MAb 2C7 was obtained. The specificity of this MAb was verified by flow cytometry, Western blotting, and competition with anti-ICOS MAb ISA3. This MAb could well recognize ICOS molecule expressed on activated T cells and induce the activation as well as proliferation of T cells prestimulated by anti-human CD3 MAb. Furthermore, we found that MAb 2C7 could induce the growth arrest of XG2 cells, a human multiple myeloma cell line, which abnormally expressed ICOS molecule, and led to its apoptosis after 48 h of treatment. This functional anti-human ICOS MAb provides a valuable tool for further study of biological functions as well as signal transduction of ICOS/GL50.
诱导性共刺激分子（ICOS）是CD28/CD152受体家族的第三个成员，是免疫反应过程中的一种重要共刺激分子。在本研究中，获得了一种具有功能的抗人ICOS单克隆抗体2C7。通过流式细胞术、蛋白质印迹法以及与抗ICOS单克隆抗体ISA3竞争，验证了该单克隆抗体的特异性。该单克隆抗体能够很好地识别活化T细胞上表达的ICOS分子，并诱导由抗人CD3单克隆抗体预刺激的T细胞的活化和增殖。此外，我们发现单克隆抗体2C7能够诱导异常表达ICOS分子的人多发性骨髓瘤细胞系XG2细胞的生长停滞，并在处理48小时后导致其凋亡。这种具有功能的抗人ICOS单克隆抗体为进一步研究ICOS/GL50的生物学功能以及信号转导提供了有价值的工具。
