[乳腺癌患者前哨淋巴结淋巴细胞亚群的初步研究]

[Preliminary study on lymphocyte subsets of sentinel lymph nodes in breast cancer patients].

作者信息

Wang Shui, Fan Ping, Wu Zheng-yan

机构信息

Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2004 Apr;26(4):220-2.

PMID:15312384

Abstract

OBJECTIVE

To study the lymphocyte subsets of sentinel lymph nodes (SLN) in breast cancer patients.

METHODS

Flow cytometry was used to analyze the markers on the surface of lymphocytes such as CD3, CD4, CD8, CD16 and CD19 in the sentinel lymph node of breast cancer.

RESULTS

When lymph node metastasis did not occur, there was no significant difference in the number of CD3(+) T, CD4(+) T, CD8(+) T, CD16 NK and CD19 B cells between SLN cells and non-SLN cells. With lymph node metastasis, the proportion of CD4(+) and CD8(+) T cells was significantly changed, CD8(+) T cells (66.15 +/- 5.97) were the predominant cells instead of CD4(+) T cells (69.07 +/- 5.02), whereas no significant difference in CD3(+) T, CD16 NK and CD19 B cells.

CONCLUSION

The CD4(+) to CD8(+) T cell ratio in sentinel lymph nodes with metastasis is reversed in breast cancer patients. This might results from changes in microenvironment due to tumor cell invasion.

摘要

目的

研究乳腺癌患者前哨淋巴结（SLN）的淋巴细胞亚群。

方法

采用流式细胞术分析乳腺癌前哨淋巴结中淋巴细胞表面标志物，如CD3、CD4、CD8、CD16和CD19。

结果

未发生淋巴结转移时，前哨淋巴结细胞与非前哨淋巴结细胞中CD3(+)T、CD4(+)T、CD8(+)T、CD16自然杀伤（NK）细胞和CD19 B细胞数量无显著差异。发生淋巴结转移时，CD4(+)和CD8(+)T细胞比例发生显著变化，CD8(+)T细胞（66.15±5.97）成为主要细胞，而非CD4(+)T细胞（69.07±5.02），而CD3(+)T、CD16 NK细胞和CD19 B细胞无显著差异。