Liu Wang, Jiang You-Hui, Li You-Liang, Lin Zi-Hao, Jiang Hua, Tan Qian, Zhang Jian-Lin, Wang Zi-Min
Department of Plastic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P. R. China.
Zhonghua Shao Shang Za Zhi. 2004 Apr;20(2):85-7.
To explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts.
Tissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts.
The points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples.
p53 gene mutation might play an important role in the formation and development of keloids.
探讨瘢痕疙瘩成纤维细胞中p53基因第4至8外显子突变的意义及作用。
分别采集12例瘢痕疙瘩患者和12例增生性瘢痕患者的组织样本用于成纤维细胞的体外培养，以同一患者的正常皮肤样本作为对照。采用基于聚合酶链反应的单链构象多态性（PCR-SSCP）和DNA测序检测成纤维细胞的p53基因突变。
12例瘢痕疙瘩组织样本中有9例检测到p53基因第4、5、6、7外显子的点突变和移码突变。在所有增生性瘢痕和正常皮肤样本中均未检测到p53基因突变。
p53基因突变可能在瘢痕疙瘩的形成和发展中起重要作用。
