脱氧核酶作为调控早期生长反应因子-1（Egr-1）基因表达的分子媒介。

DNAzymes as molecular agents that manipulate Egr-1 gene expression.

作者信息

Khachigian Levon M

机构信息

Department of Haematology, Centre for Vascular Research, University of New South Wales, Prince of Wales Hospital, Sydney, Australia.

出版信息

Biochem Pharmacol. 2004 Sep 15;68(6):1023-5. doi: 10.1016/j.bcp.2004.03.042.

DOI:10.1016/j.bcp.2004.03.042

PMID:15313396

Abstract

In recent years, the arsenal of small-molecule synthetic nucleic acids as gene-specific "knock-down" agents has increased in scope and variety. The investigator has the choice of antisense oligonucleotides, ribozymes, siRNA and DNAzymes, each subclass further benefiting from modifications that increase stability and efficiency and decrease toxicity. This review describes our use of DNAzymes in efforts to define the roles of key transcription factor targets, first in cultured vascular cells, then in animal models of neovascularization and arterial thickening.

摘要

近年来，作为基因特异性“敲低”试剂的小分子合成核酸库在范围和种类上都有所增加。研究人员可以选择反义寡核苷酸、核酶、小干扰RNA（siRNA）和脱氧核酶，每个亚类都因修饰而进一步受益，这些修饰提高了稳定性和效率，并降低了毒性。本综述描述了我们使用脱氧核酶来确定关键转录因子靶点的作用，首先是在培养的血管细胞中，然后是在新生血管形成和动脉增厚的动物模型中。