Jacquemart I, Pamuła E, De Cupere V M, Rouxhet P G, Dupont-Gillain Ch C
Unité de chimie des interfaces, Université catholique de Louvain, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium.
J Colloid Interface Sci. 2004 Oct 1;278(1):63-70. doi: 10.1016/j.jcis.2004.05.040.
The supramolecular organization of collagen adsorbed from a 7 microg/ml solution on polystyrene was investigated as a function of the adsorption duration (from 1 min to 24 h) and of the drying conditions (fast drying under a nitrogen flow, slow drying in a water-saturated atmosphere). The morphology of the created surfaces was examined by atomic force microscopy (AFM), while complementary information regarding the adsorbed amount and the organization of the adsorbed layers was obtained using radioassays, X-ray photoelectron spectroscopy (XPS), and wetting measurements. The collagen adsorbed amount increased up to an adsorption duration of 5 h and then leveled off at a value of 0.9 microg/cm2. For samples obtained by fast drying, modeling of the N/C ratios obtained by XPS in terms of thickness and surface coverage, in combination with the adsorbed amount, water contact angle measurements and AFM images, indicated that the adsorbed layer formed a felt starting from 30 min of adsorption, the density and/or the thickness of which increased with the adsorption time. Upon slow drying, the collagen layers formed after adsorption times up to about 2 h underwent a strong reorganization. The obtained nanopatterns were attributed to dewetting, the liquid film being ruptured and adsorbed collagen being displaced by the water meniscus. At higher adsorption times, the organization of the collagen layer was similar to that obtained after fast drying, because the onset of dewetting and/or collagen displacement were prevented by the high density of the collagen felt.
研究了从7微克/毫升溶液中吸附在聚苯乙烯上的胶原蛋白的超分子组织，它是吸附持续时间（从1分钟到24小时）和干燥条件（在氮气流下快速干燥、在水饱和气氛中缓慢干燥）的函数。通过原子力显微镜（AFM）检查所形成表面的形态，同时使用放射性测定、X射线光电子能谱（XPS）和润湿性测量获得关于吸附量和吸附层组织的补充信息。胶原蛋白吸附量在吸附持续时间达到5小时之前增加，然后稳定在0.9微克/平方厘米的值。对于通过快速干燥获得的样品，根据厚度和表面覆盖率对XPS获得的N/C比进行建模，并结合吸附量、水接触角测量和AFM图像表明，吸附层从吸附30分钟开始形成毡状物，其密度和/或厚度随吸附时间增加。在缓慢干燥时，吸附时间长达约2小时后形成的胶原层经历了强烈的重组。所获得的纳米图案归因于去湿，液膜破裂，吸附的胶原蛋白被水弯月面取代。在更长的吸附时间下，胶原层的组织与快速干燥后获得的相似，因为胶原毡的高密度阻止了去湿和/或胶原蛋白置换的开始。
