Xu Wen-Hui, Ye Qi-Fa, Xia Sui-Sheng
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hepatobiliary Pancreat Dis Int. 2004 Aug;3(3):428-32.
In orthotopic liver transplantation, ischemic-reperfusion is one of the most important factors that cause the incidence of biliary compliance. The aim of this study was to investigate the effects of ischemia reperfusion on epithelial cells apoptosis and proliferation of intrahepatic bile duct (IBD) (>20 microm).
30-minute warm ischemia was applied to rat livers respectively, and experiment was performed on days 2, 7, 14, 28 after reperfusion. Apoptosis was determined in situ by morphology and TUNEL, and cholangiocyte proliferation was evaluated in situ by morphometry of liver sections stained for cytokeratin-19 (CK-19) and by proliferating cellular nuclear antigen staining in liver sections.
Two days after ischemia reperfusion, apoptosis of cells was observed in large intrahepatic bile ducts (>20 microm) (5.6%+/-1.2%), but the number of large intrahepatic bile ducts reduced (0.32+/-0.06). Seven days after ischemia reperfusion, the apoptosis index of cholangiocytes decreased to 1.2%+/-0.3%, and the number of intrahepatic bile ducts began to proliferate and returned to nearly normal on day 28.
Ischemia reperfusion causes a decrease in the number of intrahepatic bile ducts (>20 microm) as a result of a higher rate of apoptosis and absence of initial proliferation.
在原位肝移植中，缺血再灌注是导致胆管并发症发生率的最重要因素之一。本研究的目的是探讨缺血再灌注对肝内胆管（内径>20微米）上皮细胞凋亡和增殖的影响。
分别对大鼠肝脏进行30分钟的热缺血处理，并在再灌注后的第2、7、14、28天进行实验。通过形态学和TUNEL法原位测定细胞凋亡，通过对细胞角蛋白-19（CK-19）染色的肝切片进行形态计量学分析以及对肝切片进行增殖细胞核抗原染色来原位评估胆管细胞增殖情况。
缺血再灌注后两天，在大的肝内胆管（内径>20微米）中观察到细胞凋亡（5.6%±1.2%），但大的肝内胆管数量减少（0.32±0.06）。缺血再灌注后七天，胆管细胞的凋亡指数降至1.2%±0.3%，肝内胆管数量开始增殖，并在第28天恢复至接近正常水平。
缺血再灌注由于较高的凋亡率和缺乏初始增殖，导致肝内胆管（内径>20微米）数量减少。
