细胞机械敏感性控制着黏着斑的各向异性。
Cell mechanosensitivity controls the anisotropy of focal adhesions.
作者信息
Nicolas Alice, Geiger Benjamin, Safran Samuel A
机构信息
Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel.
出版信息
Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12520-5. doi: 10.1073/pnas.0403539101. Epub 2004 Aug 16.
Abstract
Cellular adhesions are modulated by cytoskeletal forces or external stresses and adapt to the mechanical properties of the extracellular matrix. We propose that this mechanosensitivity can be driven at least in part by the elastic, cell-contractility-induced deformations of protein molecules that form the adhesion. The model accounts for observations of anisotropic growth and shrinkage of focal adhesions in the direction of the force and predicts that focal adhesions only grow within a range of force that is determined by the composition and matrix properties. This prediction is consistent with the observations of a force threshold for the appearance of elongated focal adhesions and the disruption of adhesions into fibrils on a mobile extracellular matrix. The growth dynamics is calculated and the predicted sliding of focal adhesions is consistent with several experiments.
摘要
细胞黏附受细胞骨架力或外部应力调节，并适应细胞外基质的力学特性。我们提出，这种机械敏感性至少部分可由形成黏附的蛋白质分子的弹性、细胞收缩诱导的变形驱动。该模型解释了粘着斑在力的方向上各向异性生长和收缩的观察结果，并预测粘着斑仅在由组成和基质特性决定的力范围内生长。这一预测与观察到的伸长粘着斑出现的力阈值以及在可移动细胞外基质上粘着斑断裂成纤维的现象一致。计算了生长动力学，预测的粘着斑滑动与多个实验结果一致。
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