Gozes Illana, Steingart Ruth A, Spier Avron D
Department of Clinical Biochemistry, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
J Mol Neurosci. 2004;24(1):67-72. doi: 10.1385/JMN:24:1:067.
An 8-amino-acid peptide, NAPVSIPQ (NAP), was identified as the smallest active element of activity-dependent neuroprotective protein that exhibits potent neuroprotective action. Potential signal transduction pathways include cGMP production and interference with inflammatory mechanisms, tumor necrosis factor-alpha, and MAC1-related changes. Because of its intrinsic structure, NAP might interact with extracellular proteins and also transverse membranes. NAP-associated protection against oxidative stress, glucose deprivation, and apoptotic mechanisms suggests interference with fundamental processes. This paper identifies p53, a key regulator of cellular apoptosis, as an intracellular target for NAP's activity.
一种由8个氨基酸组成的肽，即NAPVSIPQ（NAP），被确定为活性依赖神经保护蛋白的最小活性元件，该蛋白具有强大的神经保护作用。潜在的信号转导途径包括环磷酸鸟苷（cGMP）的产生以及对炎症机制、肿瘤坏死因子-α和与MAC1相关变化的干扰。由于其内在结构，NAP可能与细胞外蛋白质相互作用，也可能穿过细胞膜。NAP对氧化应激、葡萄糖剥夺和凋亡机制的相关保护作用表明其对基本过程存在干扰。本文确定细胞凋亡的关键调节因子p53是NAP活性的细胞内靶点。
