局部晚期非小细胞肺癌中bcl-2、bcl-X(L)和p53基因表达的初步评估。

The preliminary estimation of bcl-2, bcl-X(L) and p53 genes expression in locally advanced non-small cell lung cancer.

作者信息

Karczmarek-Borowska Bozenna, Filip Agata, Zdunek Małgorzata, Korobowicz Elzbieta, Wojcierowski Jacek, Korszeń-Pilecka Iwona, Furmanik Franciszek

机构信息

The Oncology Center of Lublin Province Department of Human Genetics, Medical University of Lublin, Lublin, Poland.

出版信息

Ann Univ Mariae Curie Sklodowska Med. 2003;58(1):124-31.

PMID:15314971

Abstract

Cell neoplastic transformation results from disturbances in expression of genes regulating its basic functions like cell cycle and apoptosis. Our paper presents preliminary estimation of expression of protooncogenes: bcl-2 and bcl-X(L) as well as of p53 suppressor gene in (NSCLC) non-small-cell lung cancer patients. The study comprised 16 NSCLC patients subjected to chemotherapy before operative procedure. Gene expression was evaluated on paraffin embedded specimens using in situ hybridization assay and immunohistochemical method. In the majority of examined patients, high expression of bcl-X(L) gene both at mRNA and protein level was ascertained. In the case of 10 patients (62.5%), higher p53 gene expression at mRNA level was observed, whereas higher level of P53 protein was determined only in four subjects (25%). In two of 16 cases (12.5%), protein product of bcl-2 gene was found, while in eight subjects (50%)--mRNA expression of the gene.

摘要

细胞的肿瘤转化是由调节其基本功能（如细胞周期和细胞凋亡）的基因表达紊乱所导致的。我们的论文展示了对原癌基因：bcl-2和bcl-X(L)以及p53抑癌基因在非小细胞肺癌（NSCLC）患者中的表达的初步评估。该研究包括16例在手术前接受化疗的NSCLC患者。使用原位杂交测定法和免疫组织化学方法在石蜡包埋标本上评估基因表达。在大多数接受检查的患者中，确定了bcl-X(L)基因在mRNA和蛋白质水平均有高表达。在10例患者（62.5%）中，观察到mRNA水平的p53基因表达较高，而仅在4例受试者（25%）中检测到P53蛋白水平较高。在16例中的2例（12.5%）中发现了bcl-2基因的蛋白质产物，而在8例受试者（50%）中发现了该基因的mRNA表达。