Surdacka Agata, Krawczyk Paweł, Dańko Małgorzata, Jastrzebska Iwona, Milanowski Janusz, Roliński Jacek
Department of Clinical Immunology, Medical University of Lublin, Lublin, Poland.
Ann Univ Mariae Curie Sklodowska Med. 2003;58(1):204-10.
The objective of the study was to evaluate the number of myeloid (DC1) and lymphoid (DC2) dendritic cells in malignant pleural effusion and peripheral blood of patients with lung cancer as well as to estimate changes in their presence during intrapleural interferon alpha treatment. The study comprised eight subjects including two patients treated with Roferon A. Isolated mononuclear cells were phenotyped with the following monoclonal antibodies: anti-BDCA-1 FITC and anti-BDCA-2 FITC, and analyzed using FACSCalibur flow cytometry. Very low percentage of DC2 and high BDCA-1/BDCA-2 ratio in malignant pleural effusion of patients not treated with IFN-alpha suggest intensive Th1 response probably responsible for inflammatory state maintenance and effusion constant development. The results of our study indicate that apart from the suggested antitumour activity, IFN-alpha therapy may result in unfavourable immunotolerance caused by increased DC2 activity.
本研究的目的是评估肺癌患者恶性胸腔积液和外周血中髓样（DC1）和淋巴样（DC2）树突状细胞的数量，并估计胸膜内注射α干扰素治疗期间它们数量的变化。该研究包括8名受试者，其中两名患者接受了罗扰素A治疗。分离的单核细胞用以下单克隆抗体进行表型分析：抗BDCA-1 FITC和抗BDCA-2 FITC，并使用FACSCalibur流式细胞仪进行分析。未接受α干扰素治疗的患者恶性胸腔积液中DC2的比例极低，BDCA-1/BDCA-2比值较高，这表明强烈的Th1反应可能是维持炎症状态和胸腔积液持续发展的原因。我们的研究结果表明，除了所提示的抗肿瘤活性外，α干扰素治疗可能会因DC2活性增加而导致不良的免疫耐受。
