Hu Shaoxian, Tao Deding, He Peigen
Department of Rheumatology, Institute of Molecular Medical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2004;24(3):245-6, 265. doi: 10.1007/BF02832002.
The expression of the costimulatory molecules B7/CD28 in peripheral blood mononuclear cells (PBMC) of the patients with systemic lupus erythematosus (SLE) and its relation to the pathogenesis of SLE were studied. The expression of the costimulatory molecules in PBMC in 30 patients with active SLE and 20 cases of healthy controls was detected by using the techniques of immunofluorescence and flow cytometer. The result showed that the expression percentage of CD28+, CD4+ CD28+ in T cells of PBMC from the patients with SLE decreased significantly as compared with that in healthy control group, while the expression percentage of CD80+, CD19+ CD80+ in B cells was significantly increased than that in healthy control group (P<0.01). It suggested that the abnormal expression of costimulatory molecules B7/CD28 played a role in the pathogenesis of SLE.
研究了系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中共刺激分子B7/CD28的表达及其与SLE发病机制的关系。采用免疫荧光技术和流式细胞仪检测30例活动期SLE患者和20例健康对照者PBMC中共刺激分子的表达。结果显示，SLE患者PBMC中T细胞CD28+、CD4+CD28+的表达率较健康对照组显著降低，而B细胞中CD80+、CD19+CD80+的表达率较健康对照组显著升高（P<0.01）。提示共刺激分子B7/CD28的异常表达在SLE发病机制中起作用。
