Jacob M C, Favre M, Lemarc'Hadour F, Sotto M F, Bonnefoix T, Sotto J J, Bensa J C
Laboratoire d'immunocytologie, Centre de Transfusion Sanguine de Grenoble, La tronche, France.
Am J Hematol. 1992 Jan;39(1):45-51. doi: 10.1002/ajh.2830390110.
Little is known about the role of tumor infiltrating T lymphocytes (TIL-T) in the pathogenesis of malignant diseases and collaboration between normal and malignant cells has not yet been proved. In the present work, we have investigated whether immune T lymphocytes exist in tumors invaded by B-cell non-Hodgkin's lymphoma (NHL) or Hodgkin's disease (HD). Therefore, we have studied the reactivity of the CD45RA monoclonal antibody, which discriminates between naive and memory CD4 T lymphocytes. Our results showed far lower percentages of CD4+ CD45RA+ in malignant lymphoma (30.3 +/- 15.0% in B-cell NHL, and 37.4 +/- 18.6% in HD) than in reactive hyperplasia (54.7 +/- 13.2%), leading to the conclusion of an accumulation of immune cells in tumor microenvironment. A further heterogeneity in the relative proportion of naive and memory TIL-T was also observed within lymphoma (range: 11 to 68% in B-cell NHL, 5 to 69% in HD). In B-cell NHL, it was related to histological features, as documented by the Kiel classification (P = .028), and to a stronger extent to cytological characteristics analysed with the Grenoble classification (P less than .0001): class 1 NHL, which are essentially indolent NHL displayed lower naive cells (22.2 +/- 7.4%) than class 3 NHL, which are more aggressive (40.1 +/- 16.1%). Among the monoclonal antibodies (mAb) defining the B-cell clone phenotype or activation state (CD19, CD20, CD21, CD22, CD23, CD24, CD5, CD10, CD11a, and Ki67), only CD23 (P = .0003) and Ki67 (P = .0007) revealed statistical association with the percentage of naive CD4 lymphocytes. No correlation could be demonstrated with the proportion of whole TIL-T, activated CD3 DR TIL-T, or CD4 subset.
关于肿瘤浸润性T淋巴细胞（TIL-T）在恶性疾病发病机制中的作用，人们了解甚少，而且正常细胞与恶性细胞之间的协作尚未得到证实。在本研究中，我们调查了B细胞非霍奇金淋巴瘤（NHL）或霍奇金病（HD）侵袭的肿瘤中是否存在免疫T淋巴细胞。因此，我们研究了CD45RA单克隆抗体的反应性，该抗体可区分初始CD4 T淋巴细胞和记忆CD4 T淋巴细胞。我们的结果显示，恶性淋巴瘤中CD4 + CD45RA + 的百分比（B细胞NHL中为30.3±15.0%，HD中为37.4±18.6%）远低于反应性增生（54.7±13.2%），由此得出肿瘤微环境中免疫细胞积聚的结论。在淋巴瘤中还观察到初始TIL-T和记忆TIL-T相对比例的进一步异质性（范围：B细胞NHL中为11%至68%，HD中为5%至69%）。在B细胞NHL中，这与组织学特征相关（如Kiel分类所记录，P = 0.028），在更大程度上与用格勒诺布尔分类分析的细胞学特征相关（P小于0.0001）：1类NHL基本为惰性NHL，其初始细胞（22.2±7.4%）低于3类NHL，后者更具侵袭性（40.1±16.1%）。在定义B细胞克隆表型或激活状态的单克隆抗体（mAb）（CD19、CD20、CD21、CD22、CD23、CD24、CD5、CD10、CD11a和Ki67）中，只有CD23（P = 0.0003）和Ki67（P = 0.0007）显示与初始CD4淋巴细胞百分比有统计学关联。未发现与整个TIL-T、活化的CD3 DR TIL-T或CD4亚群的比例有相关性。
