Diaz J I, Edinger M G, Stoler M, Tubbs R R
Department of Pathology, Cleveland Clinic Foundation, OH.
Leuk Lymphoma. 1993 Jan;9(1-2):85-90. doi: 10.3109/10428199309148508.
Tumor-infiltrating T lymphocytes (TIL-T) were quantitated by three color flow cytometry in cell suspensions from excisional biopsy specimens of 43 B cell non-Hodgkin's lymphomas (NHL) and 8 benign lymphoid hyperplasias (BLH) to identify potential differences in host T cell responses. We quantitated three TIL-T subsets: CD3+CD4+CD8- (helper-inducer), CD3+CD4-CD8+ (suppressor-cytotoxic) and CD3+CD25-HLADr+ (long term activated TIL-T) and compared them in three diagnostic groups: BLH, low grade B cell NHL (LG NHL) and intermediate-high grade B cell NHL (IG-HG NHL). The following results were obtained: Mean percentage +/- s.e. of activated TIL-T for BLH, LG-NHL and IG-HG NHL: 10.3 +/- 1.9, 23.2 +/- 4.6 and 38.8 +/- 9.5, respectively. Mean percentage +/- s.e. of suppressor-cytotoxic TIL-T for same groups: 13.9 +/- 1.5, 14.9 +/- 1.9 and 34.4 +/- 4.5, respectively. Mean percentage +/- s.e. of helper-inducer TIL-T cells for the same groups was 38.2 +/- 12.7, 32.1 +/- 7.2 and 22.5 +/- 4.6, respectively. Helper/suppressor ratio +/- s.e. for same groups was 3.0 +/- 1.1, 2.4 +/- 0.6 and 1.3 +/- 0.4, respectively. Activated and suppressor-cytotoxic TIL-T percentage progressively increased from BLH toward IG-HG NHL. The percentage of these two TIL-T subsets were significantly higher in IG-HG NHL than in BLH and LG-NHL (P < 0.0007, 0.0002, 0.0001 and 0.0260 for the comparisons TIL-T in BLH vs IG-HG NHL and LG-NHL vs IG-HG respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
通过三色流式细胞术对43例B细胞非霍奇金淋巴瘤（NHL）切除活检标本和8例良性淋巴组织增生（BLH）的细胞悬液中的肿瘤浸润性T淋巴细胞（TIL-T）进行定量分析，以确定宿主T细胞反应的潜在差异。我们对三个TIL-T亚群进行了定量分析：CD3+CD4+CD8-（辅助诱导型）、CD3+CD4-CD8+（抑制细胞毒性型）和CD3+CD25-HLADr+（长期活化的TIL-T），并在三个诊断组中进行比较：BLH、低度B细胞NHL（LG NHL）和中高度B细胞NHL（IG-HG NHL）。结果如下：BLH、LG-NHL和IG-HG NHL中活化TIL-T的平均百分比±标准误分别为：10.3±1.9、23.2±4.6和38.8±9.5。相同组中抑制细胞毒性TIL-T的平均百分比±标准误分别为：13.9±1.5、14.9±1.9和34.4±4.5。相同组中辅助诱导型TIL-T细胞的平均百分比±标准误分别为：38.2±12.7、32.1±7.2和22.5±4.6。相同组的辅助/抑制比±标准误分别为：3.0±1.1、2.4±0.6和1.3±0.4。活化和抑制细胞毒性TIL-T百分比从BLH到IG-HG NHL逐渐增加。这两个TIL-T亚群的百分比在IG-HG NHL中显著高于BLH和LG-NHL（BLH与IG-HG NHL以及LG-NHL与IG-HG NHL比较时，TIL-T的P值分别<0.0007、0.0002、0.0001和0.0260）。（摘要截短于250字）
