Salvatori F, Talassi C B, Salzgeber S A, Spinosa H S, Bernardi M M
Faculty of Veterinary Medicine, Health Sciences Institute, Paulista University, São Paulo, Brazil.
Neurotoxicol Teratol. 2004 Sep-Oct;26(5):673-80. doi: 10.1016/j.ntt.2004.05.001.
The present study was performed to evaluate the long-term behavioral effect in offspring of a subteratogenic Cd dose administered by the oral route to Wistar rat during organogenesis. First, the teratogenic Cd dose was determined by treating pregnant rats with 20 mg/kg Cd from Day 6 to Day 14 of pregnancy and by visceral and skeletal analysis of their fetuses. In a second experiment, pregnant rats treated with this Cd dose were allowed to give birth and nurture their offspring. The physical and behavioral parameters of the offspring were analyzed in infancy and during adulthood. Results showed that Cd treatment during organogenesis (1) was not able to induce maternal toxicity; (2) induced external malformations; (3) increased significantly fetus anomalies and malformations, with reduced metacarpus ossification, cleft palate and right or left renal cavitation being observed in these animals; (4) did not modify pup body weight or weight gain during the lactation period; (5) improved testis descent and delayed the vaginal opening of pups; (6) did not modify ear unfolding, incisor eruption, eye opening, negative geotaxis or palmar grasp; (7) did not modify the open-field parameters and the stereotyped behavior of male or female pups; and (8) modified male sexual behavior and (9) reduced female sexual behavior. We conclude that prenatal exposure to a teratogenic Cd dose induced in the survivor animals several deleterious effects in their development as well as in adult behaviors, mainly in the sexual sphere.
本研究旨在评估在器官形成期经口给予Wistar大鼠亚致畸剂量镉后，对其后代长期行为的影响。首先，通过在妊娠第6天至第14天用20 mg/kg镉处理怀孕大鼠，并对其胎儿进行内脏和骨骼分析来确定致畸镉剂量。在第二个实验中，用该镉剂量处理的怀孕大鼠分娩并哺育其后代。对后代的身体和行为参数在婴儿期和成年期进行了分析。结果表明，器官形成期的镉处理：（1）未诱导母体毒性；（2）诱导了外部畸形；（3）显著增加了胎儿异常和畸形，这些动物出现掌骨骨化减少、腭裂以及右肾或左肾空洞；（4）未改变哺乳期幼崽的体重或体重增加；（5）改善了睾丸下降并延迟了幼崽的阴道开口；（6）未改变耳展开、门齿萌出、睁眼、负趋地性或手掌抓握；（7）未改变雄性或雌性幼崽的旷场参数和刻板行为；（8）改变了雄性性行为；（9）降低了雌性性行为。我们得出结论，产前暴露于致畸剂量的镉会在存活动物的发育以及成年行为中，主要是在性方面，诱导多种有害影响。
