Song Mingchen, Winchester James, Albright Robert L, Capponi Vincent J, Choquette Michael D, Kellum John A
The MANTRA Laboratories, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
Blood Purif. 2004;22(5):428-34. doi: 10.1159/000080235. Epub 2004 Aug 13.
BACKGROUND/AIMS: We sought to characterize a novel adsorbent polymer in terms of cytokine removal.
We challenged 50 rats with lipopolysaccharide to obtain cytokine-rich blood and circulated this through cartridges containing polymer. In separate experiments, cell-free supernatants were passed through cartridges containing polymer. We measured tumor necrosis factor alpha, interleukin 10 and interleukin 6 concentrations under a variety of conditions to evaluate adsorption kinetics.
All three cytokines were rapidly removed from the blood with less than 50% of the initial concentrations present after 1 h of circulation through the cartridge. There was no significant difference in the effect across a range of blood flows and Ca2+ concentrations. Adsorption was decreased somewhat by extremely low temperature (4 degrees C).
The adsorbent polymer removes cytokines with high efficiency, and binding is relatively unaffected by a variety of physical conditions.
背景/目的：我们试图对一种新型吸附聚合物的细胞因子清除特性进行表征。
我们用脂多糖刺激50只大鼠以获取富含细胞因子的血液，并使其通过装有聚合物的柱管循环。在单独的实验中，无细胞上清液通过装有聚合物的柱管。我们在各种条件下测量肿瘤坏死因子α、白细胞介素10和白细胞介素6的浓度，以评估吸附动力学。
所有三种细胞因子都能从血液中迅速清除，通过柱管循环1小时后，初始浓度的50%以下仍存在。在一系列血流和钙离子浓度范围内，效果无显著差异。极低温度（4摄氏度）会使吸附略有下降。
这种吸附聚合物能高效清除细胞因子，且结合相对不受多种物理条件的影响。
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