Raziuddin S, Shetty S, Ibrahim A
Department of Clinical Immunology, King Saud University, College of Medicine, Abha, Saudi Arabia.
Eur J Immunol. 1992 Feb;22(2):309-14. doi: 10.1002/eji.1830220204.
In humans the majority of the CD3+ T cells usually express an alpha/beta T cell receptor (TcR) and a minority express a gamma/delta TcR. The CD3+ TcR alpha/beta and CD3+ TcR gamma/delta cells from blood of the patients with schistosomiasis with carcinoma of the urinary bladder (SCB) were analyzed for phenotype, activation, secretion of interleukin 2 (IL 2). B cell growth factor (BCGF) and B cell differentiation factor (BCDF), as well as for autologous (AMLR) and allogeneic (MLR) mixed lymphocyte reaction. Patients with SCB had a highly increased percentage of CD3+ TcR gamma/delta and a decreased percentage of CD3+ TcR alpha/beta T cells in their circulation. These CD3+ TcR gamma/delta T cells expressed the CD25 (IL 2 receptor), CD38, CD71 (transferrin receptor) and HLA-DR activation antigens at a higher intensity after in vitro stimulation with recombinant IL 2, phytohemagglutinin and soluble egg antigen (from Schistosoma haematobium). The SCB patients' CD3+ TcR gamma/delta T cells were highly deficient in secretion of IL 2 but produced highly elevated levels of BCGF and BCDF. On the contrary, both BCGF and BCDF activities of the CD3+ TcR alpha/beta T cells were decreased. Moreover, CD3+ TcR gamma/delta T cells demonstrated highly deficient AMLR and MLR activity. These observations suggest a possible role of CD3+ TcR gamma/delta T cells in the immune response and the disease pathogenesis in human schistosomiasis infections.
在人类中，大多数CD3⁺ T细胞通常表达α/β T细胞受体（TcR），少数表达γ/δ TcR。对患有血吸虫病合并膀胱癌（SCB）患者血液中的CD3⁺ TcR α/β和CD3⁺ TcR γ/δ细胞进行了表型、活化、白细胞介素2（IL-2）分泌、B细胞生长因子（BCGF）和B细胞分化因子（BCDF）的分析，以及自体（AMLR）和异体（MLR）混合淋巴细胞反应的分析。SCB患者循环中CD3⁺ TcR γ/δ细胞的百分比显著增加，而CD3⁺ TcR α/β T细胞的百分比降低。在用重组IL-2、植物血凝素和可溶性虫卵抗原（来自埃及血吸虫）进行体外刺激后，这些CD3⁺ TcR γ/δ T细胞以更高的强度表达CD25（IL-2受体）、CD38、CD71（转铁蛋白受体）和HLA-DR活化抗原。SCB患者的CD3⁺ TcR γ/δ T细胞IL-2分泌严重不足，但BCGF和BCDF的产生水平显著升高。相反，CD3⁺ TcR α/β T细胞的BCGF和BCDF活性均降低。此外，CD3⁺ TcR γ/δ T细胞表现出严重不足的AMLR和MLR活性。这些观察结果提示CD3⁺ TcR γ/δ T细胞在人类血吸虫病感染的免疫反应和疾病发病机制中可能发挥作用。
