Homandberg G A, Ummadi V, Kang H
Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Box 9037, Grand Forks, ND 58202, USA.
Inflamm Res. 2004 Aug;53(8):396-404. doi: 10.1007/s00011-004-1276-y. Epub 2004 Aug 10.
To test whether hyaluronan (HA) restores proteoglycan (PG) in fibronectin fragment (Fn-f) damaged cartilage through activities of insulin-like growth factor 1 (IGF-1).
Bovine cartilage explants were studied.
Cartilage PG content was measured by a dye binding assay. PG synthesis was measured by incorporation of radiolabeled sulfate. Release of IGF-1 from explants was measured by ELISA.
Higher mass HA forms were more reparative than smaller and enhanced PG synthesis in explants, but not in monolayer cultures. Higher mass HA enhanced PG synthesis and retention of IGF-1 within the explants while IGF-1 binding proteins decreased activities of HA.
The reparative effects of HA appear to involve enhanced PG synthesis through IGF-1 and requires cartilage matrix.
测试透明质酸（HA）是否通过胰岛素样生长因子1（IGF-1）的活性来恢复纤连蛋白片段（Fn-f）损伤软骨中的蛋白聚糖（PG）。
研究牛软骨外植体。
通过染料结合试验测量软骨PG含量。通过掺入放射性标记的硫酸盐来测量PG合成。通过酶联免疫吸附测定法测量外植体中IGF-1的释放。
较高质量的HA形式比较小的HA更具修复性，并增强了外植体中的PG合成，但在单层培养中则不然。较高质量的HA增强了PG合成以及IGF-1在外植体内的保留，而IGF-1结合蛋白则降低了HA的活性。
HA的修复作用似乎涉及通过IGF-1增强PG合成，并且需要软骨基质。
