Khaldi Nadia, Miras Alain, Botti Koffi, Benali Larbi, Gromb Sophie
Forensic Science Laboratory, Pellegrin Hospital, University Teaching Hospital Bordeaux, Bordeaux Cedex, France.
J Forensic Sci. 2004 Jul;49(4):749-53.
We sought to discover whether spermatozoa concentration and the delay between ejaculation and test influence the results of seminal fluid fast detection tests. Two hundred and twenty-seven anonymous samples divided into four groups (normospermia, oligospermia, azoospermia, and controls) after a semen analysis were subjected to three fast detection semen tests: Diff-Quick fast coloration, Phosphatesmo Km Paper for acid phosphatases (AP) detection, and PSA-Check 1 for prostate specific antigen (PSA) detection. The study was performed at three time points (0, 48, and 72 h). Unlike cytology, results obtained with AP and PSA were not influenced by spermatozoa concentration. PSA detection results remained constant up to 72 h and were more reliable after 48 h than those obtained by AP detection.
我们试图探究精子浓度以及射精与检测之间的时间间隔是否会影响精液快速检测试验的结果。在进行精液分析后，将227份匿名样本分为四组（正常精子症、少精子症、无精子症和对照组），并对其进行三种精液快速检测：Diff-Quick快速染色、用于酸性磷酸酶（AP）检测的磷酸盐Km试纸以及用于前列腺特异性抗原（PSA）检测的PSA-Check 1。该研究在三个时间点（0、48和72小时）进行。与细胞学不同，AP和PSA检测结果不受精子浓度的影响。PSA检测结果在72小时内保持恒定，且在48小时后比AP检测结果更可靠。
