van Heerden J, Ehlers M M, van Zyl W B, Grabow W O K
Dept of Medical Virology, University of Pretoria, Pretoria 0001, South Africa.
Water Sci Technol. 2004;50(1):39-43.
Human adenoviruses (HAds), of which there are 51 antigenic types, are associated aetiologically with gastrointestinal, respiratory, urinary tract and eye infections. The clinical importance of HAds and the potential health risks constituted by HAds in water environments are widely recognised. This study was conducted to assess the use of an optimised integrated cell culture molecular-based technique to determine the prevalence of HAds in raw and treated drinking-water supplies in South Africa. Selected supplies were monitored weekly for the presence of adenoviruses over a one-year period (July 2001 to June 2002). Drinking-water supplies were derived from acceptable quality surface water sources using treatment processes that conformed to international standards for the production of safe drinking water. Adenoviruses were detected by amplification in cell cultures, followed by amplifying the extracted nucleic acids using molecular techniques (nested PCR). HAds were detected in 29.8% (59/198) of the treated drinking water, 16% (8/50) of dam water and 44% (22/50) of river-water samples tested. The results of this study confirmed the presence of HAds in some raw and treated drinking water supplies in South Africa.
人类腺病毒（HAds）共有51种抗原型，在病因上与胃肠道、呼吸道、泌尿道和眼部感染有关。HAds的临床重要性以及水环境中HAds构成的潜在健康风险已得到广泛认可。本研究旨在评估使用优化的基于细胞培养分子技术来确定南非原水和处理后饮用水供应中HAds的流行情况。在一年时间（2001年7月至2002年6月）内，每周对选定的供水进行腺病毒检测。饮用水供应取自质量合格的地表水水源，采用符合国际安全饮用水生产标准的处理工艺。通过细胞培养扩增检测腺病毒，然后使用分子技术（巢式PCR）扩增提取的核酸。在所检测的处理后饮用水样本中，29.8%（59/198）检测到HAds，在坝水样本中16%（8/50）检测到，在河水样本中44%（22/50）检测到。本研究结果证实南非一些原水和处理后饮用水供应中存在HAds。
