视网膜母细胞瘤易感基因产物在细胞周期进入和进展的多个阶段发生磷酸化。
The retinoblastoma-susceptibility gene product becomes phosphorylated in multiple stages during cell cycle entry and progression.
作者信息
DeCaprio J A, Furukawa Y, Ajchenbaum F, Griffin J D, Livingston D M
机构信息
Dana-Farber Cancer Institute, Boston, MA 02115.
出版信息
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1795-8. doi: 10.1073/pnas.89.5.1795.
Abstract
The retinoblastoma-susceptibility gene product (RB) undergoes cell cycle-dependent phosphorylation and dephosphorylation. We characterized RB phosphorylation after mitogenic stimulation of primary human T lymphocytes, initially arrested in the G0 state. RB is phosphorylated in at least three steps when T cells are driven into the cell cycle. The first event occurs during mid G1 phase, the second during S phase, and the third in G2/M. Tryptic phosphopeptide mapping indicates that the different phosphorylation events occur, at least in part, on different residues in RB. Given the known relationship of the RB phosphorylation state to function, it is possible that RB regulates growth at multiple points in the cell cycle.
摘要
视网膜母细胞瘤易感基因产物（RB）经历细胞周期依赖性磷酸化和去磷酸化过程。我们对最初停滞于G0期的原代人T淋巴细胞进行有丝分裂原刺激后的RB磷酸化情况进行了表征。当T细胞进入细胞周期时，RB至少在三个步骤中发生磷酸化。第一个事件发生在G1期中期，第二个发生在S期，第三个发生在G2/M期。胰蛋白酶磷酸肽图谱分析表明，不同的磷酸化事件至少部分发生在RB的不同残基上。鉴于RB磷酸化状态与功能之间的已知关系，RB有可能在细胞周期的多个点调节生长。
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