Gurbuz N, Yalcin O, Aksu T A, Baskurt O K
Department of Biochemistry, Akdeniz University Faculty of Medicine, Antalya, Turkey.
Clin Hemorheol Microcirc. 2004;31(3):235-42.
Glucose-6-phosphate dehydrogenase (G6PD) activity, red blood cell (RBC) lipid peroxidation and deformability were investigated in hemizygous and heterozygous G6PD deficient subjects and compared with normal individuals. None of the subjects were in acute hemolytic crises. G6PD activity was assessed based on the spectrophotometric determination of generated NADPH. Lipid peroxidation was measured as thiobarbutiric acid reactive substances (TBARS). RBC deformability was analyzed by ektacytometry. RBC lipid peroxidation was found to be significantly higher in hemizygous subjects compared to control and heterozygous subjects, while RBC deformability was found to be significantly impaired. However, although lipid peroxidation was higher than control, RBC deformability was not significantly different from control in heterozygous individuals, characterized by significantly lower RBC G6PD activity. There were no significant correlations between these three parameters when the three groups were analyzed separately, but a significant negative correlation was found to exist between G6PD activity and TBARS when the pooled data from the three groups were used for the analysis. This was also true for the relationship between RBC deformability and G6PD activity. It has been concluded that G6PD activity is not a good predictor of oxidative damage resulting in mechanical impairment in heterozygous individuals.
对半合子和杂合子葡萄糖-6-磷酸脱氢酶（G6PD）缺乏症患者的G6PD活性、红细胞（RBC）脂质过氧化和变形性进行了研究，并与正常个体进行了比较。所有受试者均未处于急性溶血危机中。基于分光光度法测定生成的NADPH来评估G6PD活性。脂质过氧化以硫代巴比妥酸反应性物质（TBARS）来衡量。通过激光衍射法分析RBC变形性。发现半合子受试者的RBC脂质过氧化显著高于对照组和杂合子受试者，而RBC变形性则显著受损。然而，尽管脂质过氧化高于对照组，但杂合子个体的RBC变形性与对照组无显著差异，其特征是RBC G6PD活性显著降低。当分别分析这三组时，这三个参数之间没有显著相关性，但当使用三组的汇总数据进行分析时，发现G6PD活性与TBARS之间存在显著的负相关。RBC变形性与G6PD活性之间的关系也是如此。得出的结论是，G6PD活性不是杂合子个体中导致机械损伤的氧化损伤的良好预测指标。
