Suzuki Yasuo, Hattori Shunji, Isemura Mamoru
School of Food and Nutritional Sciences, and Center of Excellence for the 21st Century, University of Shizuoka, University of Shizuoka, Yada, Shizuoka 422-8526, Japan.
Biosci Biotechnol Biochem. 2004 Aug;68(8):1817-20. doi: 10.1271/bbb.68.1817.
The effects of (-)-epigallocatechin gallate (EGCG) on the contraction of floating collagen gel by fibroblasts were investigated. EGCG inhibited collagen gel contraction dose-dependently. On the basis of the fact that platelet-derived growth factor (PDGF) is one of the serum components with stimulatory activity in collagen gel contraction, we examined the possibility that interaction between EGCG and PDGF may be involved in this inhibition mechanism. We confirmed this by recombinant PDGF-BB in the present system and we found that EGCG inhibited PDGF-stimulated collagen gel contraction. The results of affinity chromatography indicated that PDGF was bound by EGCG immobilized on agarose gel as detected by enzyme-linked immunoassay and Western blotting. These findings suggest that binding of EGCG to PDGF is at least partly involved in the mechanism of inhibition of collagen gel contraction by EGCG.
