Vitanova Lily, Haverkamp Silke, Wässle Heinz
Department of Physiology, Medical University, Sofia, Bulgaria.
Cell Tissue Res. 2004 Sep;317(3):227-35. doi: 10.1007/s00441-004-0914-6. Epub 2004 Jul 29.
Glycine and glycine receptors (GlyRs) were analyzed immunocytochemically in the retina of the frog Rana ridibunda. Glycine was localized to somata of glycinergic amacrine and interplexiform cells. Approximately 50% of the cells in the amacrine cell layer were found to be glycinergic. GlyRs of the inner plexiform layer (IPL) were localized to brightly fluorescent puncta, probably representing postsynaptic clusters of GlyRs. GlyR clusters were not evenly distributed across the IPL but showed patterns of stratification specific for the various GlyR subunits. Clusters containing the alpha1 subunit formed four narrow strata within the IPL. Clusters containing the alpha3 subunit were more abundant and covered the whole IPL, with a band of higher density in stratum 3. Clusters of GlyRs were also observed in the outer plexiform layer. Thus, several isoforms of synaptic GlyRs involved with different synapses and inhibitory circuits are present in the frog retina.
运用免疫细胞化学方法，对泽蛙视网膜中的甘氨酸及甘氨酸受体（GlyRs）进行了分析。甘氨酸定位于甘氨酸能无长突细胞和网间细胞的胞体。发现无长突细胞层中约50%的细胞为甘氨酸能细胞。内网状层（IPL）的甘氨酸受体定位于明亮的荧光斑点，可能代表甘氨酸受体的突触后簇。甘氨酸受体簇在IPL中分布不均，但显示出各种甘氨酸受体亚基特有的分层模式。含有α1亚基的簇在IPL内形成四个狭窄的层。含有α3亚基的簇更为丰富，覆盖整个IPL，在第3层有一条高密度带。在外网状层也观察到了甘氨酸受体簇。因此，蛙视网膜中存在几种与不同突触和抑制性回路相关的突触甘氨酸受体亚型。
