Grünert U, Wässle H
Max-Planck-Institut für Hirnforschung, Frankfurt am Main, Germany.
J Comp Neurol. 1993 Sep 22;335(4):523-37. doi: 10.1002/cne.903350405.
The distribution of glycinergic synapses in the mammalian retina was studied with monoclonal antibodies against glycine receptors and a glycine receptor-related protein (gephyrin). Monoclonal antibody 2b is specific for the alpha 1 subunit of the glycine receptor; monoclonal antibody 4a is specific for all known alpha subunits and the beta subunit, and monoclonal antibody 7a is specific for gephyrin. The three antibodies were applied to the retina of cat, macaque monkey, rat, and rabbit. The general staining pattern is comparable in all these species and it is similar but distinct with all of the three antibodies. Labeling is characterized by a punctate appearance indicating that it occurs at synapses. In the inner plexiform layer, labeling is concentrated in two bands. One band is located close to the inner nuclear layer; the other band is located in the middle of the inner plexiform layer. In the outer plexiform layer, sparse punctate labeling is seen. The distribution of gephyrin was also studied at the ultrastructural level in cat and monkey retina. Gephyrin is present on the postsynaptic membrane of amacrine cells and ganglion cells. The presynaptic profile to gephyrin immunoreactivity is always of an amacrine cell. The AII amacrine cell, the crucial glycinergic interneuron of the rod pathway, is presynaptic to gephyrin immunoreactivity in the OFF-sublamina and is itself gephyrin-positive at an input synapse from another (possibly GABAergic) amacrine cell in the ON-sublamina.
利用针对甘氨酸受体和一种与甘氨酸受体相关蛋白（gephyrin）的单克隆抗体，研究了甘氨酸能突触在哺乳动物视网膜中的分布。单克隆抗体2b对甘氨酸受体的α1亚基具有特异性；单克隆抗体4a对所有已知的α亚基和β亚基具有特异性，单克隆抗体7a对gephyrin具有特异性。将这三种抗体应用于猫、猕猴、大鼠和兔的视网膜。在所有这些物种中，总体染色模式是可比的，并且与所有三种抗体相比是相似但不同的。标记的特征是点状外观，表明它发生在突触处。在内网状层中，标记集中在两条带中。一条带靠近内核层；另一条带位于内网状层的中间。在外网状层中，可以看到稀疏的点状标记。还在猫和猴视网膜的超微结构水平上研究了gephyrin的分布。Gephyrin存在于无长突细胞和神经节细胞的突触后膜上。与gephyrin免疫反应性相关的突触前轮廓总是无长突细胞。AII无长突细胞是视杆通路中关键的甘氨酸能中间神经元，在OFF亚层中是gephyrin免疫反应性的突触前细胞，并且在ON亚层中来自另一个（可能是GABA能）无长突细胞的输入突触处其本身是gephyrin阳性的。
