El-Naggar M M, Arafa S Z, El-Abbassy S A, Stewart M T, Halton D W
Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
Parasitol Res. 2004 Oct;94(3):163-75. doi: 10.1007/s00436-004-1198-1. Epub 2004 Aug 20.
Phalloidin fluorescence technique, enzyme cytochemistry and immunocytochemistry in conjunction with confocal scanning laser microscopy have been used for the first time to describe the nervous and muscle systems of the viviparous monogenean gill parasite, Macrogyrodactylus clarii. The gross spatial arrangement of muscle and associated cholinergic, peptidergic and aminergic innervations has been examined. The central nervous system (CNS) consists of paired cerebral ganglia from which emanate three pairs of longitudinal ventral, lateral and dorsal nerve cords, connected at intervals by transverse connectives. The CNS is better developed ventrally than dorsally or laterally, and has the strongest reactivity for all neuroactive substances examined. Structural and functional correlates of the neuromusculature of the pharynx, haptor and reproductive tracts have been examined. Results implicate acetylcholine, FMRFamide-related peptides (FaRPs) and serotonin in sensory and motor function in this monogenean, although confirmatory physiological data are obviously required.
首次使用鬼笔环肽荧光技术、酶细胞化学和免疫细胞化学结合共聚焦扫描激光显微镜来描述胎生单殖吸虫鳃寄生虫——克拉氏巨旋虫的神经和肌肉系统。已对肌肉以及相关的胆碱能、肽能和胺能神经支配的总体空间排列进行了研究。中枢神经系统（CNS）由成对的脑神经节组成，从这些神经节发出三对纵向的腹侧、外侧和背侧神经索，它们通过横向连接体间隔相连。中枢神经系统在腹侧比背侧或外侧发育得更好，并且对所有检测的神经活性物质具有最强的反应性。已对咽、吸器和生殖道神经肌肉组织的结构和功能相关性进行了研究。结果表明，乙酰胆碱、FMRF酰胺相关肽（FaRPs）和5-羟色胺参与了这种单殖吸虫的感觉和运动功能，尽管显然需要确证性的生理学数据。
