Lindsay Laura A, Murphy Christopher R
Department of Anatomy and Histology, The University of Sydney, NSW, Australia.
J Mol Histol. 2004 Jan;35(1):75-9. doi: 10.1023/b:hijo.0000021066.44364.81.
Immunofluorescence and immunogold techniques were used to determine the presence and distribution of aquaporin-1 (AQP1) within the rat uterus. Uterine tissue from non-pregnant (proestrus) as well as pregnant (days 1, 3, 6 and 7) rats were used. It was found that this water channel was present in the myometrium of the pregnant rat uterus with the intensity of AQP1 immunoreactivity increasing from day 1 to day 6 of pregnancy. In particular, an increase was also observed in mesometrial as compared to antimesometrial myometrium. Immunolocalization at the electron microscope level indicated that AQP1 was localized to the plasma membrane of smooth muscle cells found within the inner circular layer. It is suggested that AQP1 plays a role in stromal oedema, uterine closure and orientation of the blastocyst.
采用免疫荧光和免疫金技术来确定水通道蛋白-1（AQP1）在大鼠子宫内的存在及分布情况。使用了来自未孕（动情前期）以及孕鼠（妊娠第1、3、6和7天）的子宫组织。结果发现，这种水通道存在于妊娠大鼠子宫的肌层中，且AQP1免疫反应性强度从妊娠第1天到第6天逐渐增加。特别是，与子宫系膜对侧的肌层相比，子宫系膜侧的肌层中也观察到了增加。电子显微镜水平的免疫定位表明，AQP1定位于内环层平滑肌细胞的质膜上。提示AQP1在基质水肿、子宫闭合和囊胚定位中发挥作用。
