Frey J R, Ernst B, Surh C D, Sprent J
Department of Immunology, Scripps Research Institute, La Jolla, California 92037.
J Exp Med. 1992 Apr 1;175(4):1067-71. doi: 10.1084/jem.175.4.1067.
To seek direct evidence for the notion that stem cells in the thymus need to be constantly replenished from the bone marrow (BM), fetal (day 15) thymuses from normal BALB/c mice were grafted into T and B cell-deficient C.B-17 SCID mice (both H-2d, I-E+). The thymus grafts in these mice showed normal thymopoiesis for the first 3 wk postgrafting but then developed sudden atrophy with near complete loss of CD4+8+ cells by 4-5 wk. Such atrophy was not seen when the thymus-grafted mice were cotransplanted with normal BM cells. The lymph nodes of SCID mice receiving thymus grafts alone contained mature T cells but virtually no B cells. This lack of B cells was associated with aberrant I-E-restricted V beta deletion: the depletion of V beta 3+ and V beta 5+ T cells was near complete, whereas V beta 11+ cells showed only marginal depletion.
为了寻找胸腺中的干细胞需要不断从骨髓（BM）补充这一观点的直接证据，将正常BALB/c小鼠的胎鼠（第15天）胸腺移植到T和B细胞缺陷的C.B-17 SCID小鼠（均为H-2d，I-E+）体内。这些小鼠的胸腺移植在移植后的前3周显示出正常的胸腺生成，但随后出现突然萎缩，到4-5周时CD4+8+细胞几乎完全丧失。当胸腺移植小鼠与正常骨髓细胞共同移植时，未观察到这种萎缩。单独接受胸腺移植的SCID小鼠的淋巴结含有成熟T细胞，但几乎没有B细胞。这种B细胞的缺乏与异常的I-E限制性Vβ缺失有关：Vβ3+和Vβ5+ T细胞的耗竭几乎完全，而Vβ11+细胞仅显示出轻微的耗竭。
