McGraw Lisa A, Gibson Greg, Clark Andrew G, Wolfner Mariana F
Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Curr Biol. 2004 Aug 24;14(16):1509-14. doi: 10.1016/j.cub.2004.08.028.
In Drosophila melanogaster, sperm and accessory gland proteins ("Acps," a major component of seminal fluid) transferred by males during mating trigger many physiological and behavioral changes in females (reviewed in ). Determining the genetic changes triggered in females by male-derived molecules and cells is a crucial first step in understanding female responses to mating and the female's role in postcopulatory processes such as sperm competition, cryptic female choice, and sexually antagonistic coevolution. We used oligonucleotide microarrays to compare gene expression in D. melanogaster females that were either virgin, mated to normal males, mated to males lacking sperm, or mated to males lacking both sperm and Acps. Expression of up to 1783 genes changed as a result of mating, most less than 2-fold. Of these, 549 genes were regulated by the receipt of sperm and 160 as a result of Acps that females received from their mates. The remaining genes whose expression levels changed were modulated by nonsperm/non-Acp aspects of mating. The mating-dependent genes that we have identified contribute to many biological processes including metabolism, immune defense, and protein modification.
在黑腹果蝇中，雄性在交配过程中传递的精子和附属腺蛋白（“Acps”，精液的主要成分）会引发雌性许多生理和行为上的变化（相关综述见 ）。确定雄性来源的分子和细胞在雌性中引发的基因变化，是理解雌性对交配的反应以及雌性在诸如精子竞争、隐性雌性选择和性拮抗协同进化等交配后过程中所起作用的关键第一步。我们使用寡核苷酸微阵列比较了黑腹果蝇雌性的基因表达情况，这些雌性分别为处女蝇、与正常雄性交配的雌性、与无精子雄性交配的雌性，或与既无精子又无 Acps 的雄性交配的雌性。多达 1783 个基因的表达因交配而发生变化，大多数变化幅度小于 2 倍。其中，549 个基因受精子的影响而被调控，160 个基因受雌性从配偶那里获得的 Acps 的影响而被调控。其余表达水平发生变化的基因则受交配的非精子/非 Acp 方面因素的调节。我们鉴定出的交配依赖性基因参与了许多生物学过程，包括新陈代谢、免疫防御和蛋白质修饰。
