来自苏格兰低地养羊场的两种对三种药物耐药的捻转血矛线虫田间分离株的特性分析。
Characterisation of two triple resistant field isolates of Teladorsagia from Scottish lowland sheep farms.
作者信息
Bartley D J, Jackson F, Jackson E, Sargison N
机构信息
Department of Parasitology, Moredun Research Institute, Pentland Science Park, Bush Loan, Penicuik EH26 0PZ, UK.
出版信息
Vet Parasitol. 2004 Sep 2;123(3-4):189-99. doi: 10.1016/j.vetpar.2004.06.018.
The anthelmintic resistance status of two field isolates derived from farms (farm A and B) located near Edinburgh were examined using both controlled efficacy tests (CET) and faecal egg count reduction tests (FECRT). Efficacies against fenbendazole (FBZ), levamisole (LEV) and ivermectin (IVM) and, for one isolate, against combinations of these anthelmintics and moxidectin were determined in naïve lambs, artificially infected with the isolates and treated with the compounds at the manufacturers recommended dose rates. (FBZ, 5mg/kg bodyweight (BW); LEV, 7.5mg/kg BW; IVM, 0.2mg/kg BW; Moxidectin (MOX) 0.2mg/kg BW). In both field isolates, the predominant species found pre-treatment and the only species found post-treatment was Teladorsagia circumcincta. Resistance to FBZ, LEV and IVM was confirmed in CET and FECRT on farm A and to the latter two compounds on farm B, which had a history of benzimidazole resistance and where TBZ resistance was also demonstrated using an egg hatch assay (EHA). For the farm A isolate CET efficacies against FBZ; IVM; LEV; FBZ + IVM; FBZ + LEV; FBZ, LEV + IVM and MOX were 59, 60, 88, 94,93, 92 and 98%, respectively. The CET efficacies for the farm B isolate were 51% and 72% for LEV and IVM, respectively.
采用对照药效试验（CET）和粪便虫卵计数减少试验（FECRT），对来自爱丁堡附近农场（农场A和农场B）的两个野外分离株的驱虫抗性状况进行了检测。在初次使用的羔羊中，人工感染这些分离株并用制造商推荐剂量的化合物进行治疗，测定了对芬苯达唑（FBZ）、左旋咪唑（LEV）和伊维菌素（IVM）的药效，对于其中一个分离株，还测定了对这些驱虫药与莫西菌素组合的药效。（FBZ，5mg/kg体重（BW）；LEV，7.5mg/kg BW；IVM，0.2mg/kg BW；莫西菌素（MOX）0.2mg/kg BW）。在这两个野外分离株中，治疗前发现的主要物种以及治疗后发现的唯一物种是环形泰勒虫。在农场A的CET和FECRT中证实了对FBZ、LEV和IVM的抗性，在农场B证实了对后两种化合物的抗性，农场B有苯并咪唑抗性历史，并且使用虫卵孵化试验（EHA）也证明了对噻苯达唑的抗性。对于农场A分离株，对FBZ、IVM、LEV、FBZ + IVM、FBZ + LEV、FBZ、LEV + IVM和MOX的CET药效分别为59%、60%、88%、94%、93%、92%和98%。农场B分离株对LEV和IVM的CET药效分别为51%和72%。
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