在巴西的两种野生犬科动物——草原狐（Lycalopex gymnocercus）和食蟹狐（Cerdocyon thous）中检测犬新孢子虫抗体。
Detection of antibodies to Neospora caninum in two species of wild canids, Lycalopex gymnocercus and Cerdocyon thous from Brazil.
作者信息
Cañón-Franco W A, Yai L E O, Souza S L P, Santos L C, Farias N A R, Ruas J, Rossi F W, Gomes A A B, Dubey J P, Gennari S M
机构信息
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva, 87, Cidade Universitária São Paulo, São Paulo, CEP 05508-000, Brazil.
出版信息
Vet Parasitol. 2004 Sep 2;123(3-4):275-7. doi: 10.1016/j.vetpar.2004.06.004.
Domestic dog (Canis domesticus) and the coyote (Canis latrans) are the only known definitive hosts for the protozoan Neospora caninum that causes abortion in dairy cattle. In the present study, antibodies to N. caninum were sought in three species of wild canids, Cerdocyon thous, Lycalopex gymnocercus and Dusicyon vetulus from Brazil. Antibodies to N. caninum were assayed by the indirect fluorescent antibody test (IFAT) and the Neospora agglutination test (NAT). N. caninum antibodies were found in five of 12 L. gymnocercus with IFAT titers of 1:50 in three, 1:100 in one, and 1:1600 in one, and NAT titers of 1:40, 1:80, 1:160, 1:320, and 1:640 in five animals. Antibodies to N. caninum were found in four of 15 C. thous with IFAT titers of 1:50 in one, and 1:100 in three, and NAT titer of 1:40 in one animal. All 30 D. ventulus were seronegative by IFAT and NAT.
家犬（犬属家犬）和郊狼（犬属丛林狼）是已知的原生动物新孢子虫仅有的终末宿主，新孢子虫可导致奶牛流产。在本研究中，对来自巴西的三种野生犬科动物——食蟹狐、草原狐和南极狐，检测了新孢子虫抗体。通过间接荧光抗体试验（IFAT）和新孢子虫凝集试验（NAT）检测新孢子虫抗体。在12只草原狐中有5只检测到新孢子虫抗体，其中3只IFAT滴度为1:50，1只滴度为1:100，1只滴度为1:1600；5只动物的NAT滴度分别为1:40、1:80、1:160、1:320和1:640。在15只食蟹狐中有4只检测到新孢子虫抗体，其中1只IFAT滴度为1:50，3只滴度为1:100；1只动物的NAT滴度为1:40。所有30只南极狐通过IFAT和NAT检测均为血清阴性。
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