巴西圣保罗市南美负鼠（Didelphis marsupialis）中犬新孢子虫和刚地弓形虫抗体的血清阳性率。
Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in the South American opossum (Didelphis marsupialis) from the city of São Paulo, Brazil.
作者信息
Yai L E O, Cañon-Franco W A, Geraldi V C, Summa M E L, Camargo M C G O, Dubey J P, Gennari S M
机构信息
Centro de Controle de Zoonoses da Prefeitura do Município de São Paulo, SP, Brazil.
出版信息
J Parasitol. 2003 Aug;89(4):870-1. doi: 10.1645/GE-83R.
Antibodies to Neospora caninum and Toxoplasma gondii were assayed in sera of 396 opossums (Didelphis marsupialis) from the city of São Paulo, Brazil. Antibodies to N. caninum were assayed using the indirect immunofluorescent antibody test (IFAT). Antibodies (IFAT, approximately 1:25) to N. caninum were found in 84 opossums (D. marsupialis) in titers of 1:25 in 46, 1:50 in 20, 1:100 in 17, and 1:400 in 1. Antibodies to T. gondii were assayed with the modified agglutination test (MAT) and the IFAT. Antibodies to T. gondii (MAT, approximately 1:25) were found in 82 (20.4%) of the 396 opossums, in titers of 1:25 in 24, 1:50 in 26, 1:100 in 18, 1:200 in 13, and 1:800 in 1. The IFAT antibodies to T. gondii were found in 148 of 396 opossums, in titers of 1:16 in 41, 1:32 in 23, 1:64 in 13, 1:128 in 6, 1:256 in 20, 1:512 in 17, 1:1,024 in 10, 1:2,048 in 10, 1:4,096 in 7, and 1:8,192 in 1. This is the first report of N. caninum and T. gondii infections in D. marsupialis.
在巴西圣保罗市的396只负鼠（Didelphis marsupialis）血清中检测了抗犬新孢子虫和抗刚地弓形虫的抗体。使用间接免疫荧光抗体试验（IFAT）检测抗犬新孢子虫的抗体。在84只负鼠（D. marsupialis）中发现了抗犬新孢子虫的抗体（IFAT，约为1:25），其中46只的效价为1:25，20只的效价为1:50，17只的效价为1:100，1只的效价为1:400。用改良凝集试验（MAT）和IFAT检测抗刚地弓形虫的抗体。在396只负鼠中的82只（20.4%）中发现了抗刚地弓形虫的抗体（MAT，约为1:25），其中24只的效价为1:25，26只的效价为1:50，18只的效价为1:100，13只的效价为1:200，1只的效价为1:800。在396只负鼠中的148只中发现了IFAT抗刚地弓形虫抗体，其中41只的效价为1:16，23只的效价为1:32，13只的效价为1:64，6只的效价为1:128，20只的效价为1:256，17只的效价为1:512，10只的效价为1:1,024，10只的效价为1:2,048,7只的效价为1:4,096，1只的效价为1:8,192。这是关于犬新孢子虫和刚地弓形虫在D. marsupialis中感染的首次报告。
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