恒压灌注下摘除的猪眼葡萄膜巩膜流出物的特征
Characterization of uveoscleral outflow in enucleated porcine eyes perfused under constant pressure.
作者信息
Wagner Justin A, Edwards Aurélie, Schuman Joel S
机构信息
Department of Chemical and Biological Engineering, Tufts University, Medford, Massachusetts 02155, USA.
出版信息
Invest Ophthalmol Vis Sci. 2004 Sep;45(9):3203-6. doi: 10.1167/iovs.03-1176.
PURPOSE
A new technique was developed to measure the flow of aqueous humor through the uveoscleral pathway in porcine eyes and to examine whether there is any outflow through the choroid into the vortex veins.
METHODS
Enucleated porcine eyes were perfused in vitro under a constant pressure of 10 mm Hg. After total outflow was measured, the episcleral vessels were blocked with cyanoacrylate to eliminate outflow through the conventional pathway. The vortex veins were then blocked, to assess the amount of choroidal drainage.
RESULTS
The average outflow in control eyes was found to be 2.8 +/- 0.9 microL/min. After the exit sites of the conventional pathway were blocked, the average outflow decreased to 1.1 +/- 0.5 microL/min. Blocking the vortex veins did not appear to alter uveoscleral outflow further (1.2 +/- 0.8 microL/min).
CONCLUSIONS
The results suggest that choroidal drainage into the vortex veins is insignificant in the absence of blood perfusion. No significant washout effects in porcine eyes were observed.
目的
开发一种新技术来测量猪眼房水通过葡萄膜巩膜途径的流量，并研究是否存在房水通过脉络膜流入涡静脉的情况。
方法
摘除的猪眼在体外10毫米汞柱的恒定压力下进行灌注。在测量总流出量后，用氰基丙烯酸酯封闭巩膜上的血管以消除通过传统途径的流出。然后封闭涡静脉，以评估脉络膜引流的量。
结果
发现对照眼的平均流出量为2.8±0.9微升/分钟。在传统途径的出口部位被封闭后，平均流出量降至1.1±0.5微升/分钟。封闭涡静脉似乎并未进一步改变葡萄膜巩膜流出量（1.2±0.8微升/分钟）。
结论
结果表明，在没有血液灌注的情况下，脉络膜向涡静脉的引流微不足道。在猪眼中未观察到明显的冲洗效应。
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